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CC323-1

Sigma-Aldrich

PhotoGel-IRG, Methacrylated Gelatin Hydrogel Kit

Photocrosslinked gelatin hydrogel (CC323 Kit Component 1)

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form

lyophilized powder

application(s)

cell analysis

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CC324-1CC324-2CC320-1
application(s)

cell analysis

application(s)

cell analysis

application(s)

cell analysis

application(s)

cell analysis

General description

3D cell culture, including bioprinting, allows for the creation of more physiological cell models by allowing cells to simultaneously interact with integrins on all cell surfaces, resulting in the activation of specific signaling pathways not activated in traditional 2D cell culture methods. Hydrogels are water swollen polymers that allow for the culture of cells in 3-dimensions and can have profound effects on cellular development, differentiation, migration, and function. New areas of tissue engineering such as 3D bioprinting, have utilized UV photocrosslinked methacrylated hydrogel biomaterials (PEGMA, GelMA, HAMA and ColMA etc.) to encapsulate cells to make printable bioinks. The PhotoGel-IRG, Methacrylated Gelatin Hydrogel Kit (CC323: CC323-1, CC323-2) is based upon purified porcine gelatin methacrylate (GelMA), which when photocrosslinked provides a native-like 3D environment for cells. Gelatin derived from denatured collagen retains many natural cell binding motifs such as RGD and MMP sites. In addition to porcine gelatin methacrylate, the kit includes the photoinitiator Irgacure 2959 for users to easily fine tune their photocrosslinking experiments (i.e. altering hydrogel stiffness or gelling speeds). Gelatin methacrylate is produced from porcine, type A, 300 bloom gelatin. Gelatin macromers containing primary amino groups were reacted with methacrylic anhydride (MA) to add methacrylate pendant groups. The gelatin methacrylate achieves a degree of substitution of >70% for maximum crosslinking and range of stiffness.PhotoCol is a registered trademark of Advanced BioMatrix, Inc.

Application

Photocrosslinking is initiated using a 365 nm UV light source.

Packaging

1 Kit

Components

CC323-1 (Store at 2-8°C): Irgacure Photoinitiator, 1 X 100 mg (CS226351).
Note: The complete PhotoGel-IRG, Methacrylated Gelatin Hydrogel Kit (CC323) consists of CC323-1 and CC323-2 sold seperately.

Quality

Appearance: Lyophilized powderGelatin Content: ≥ 1 gramSterility (USP modified): No GrowthpH: 6.0-8.0Grafting Efficiency: ≥ 75%Cell Compatibility: >70% Cell Viability

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Environment

hcodes

Hazard Classifications

Aquatic Chronic 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1


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Articles

Methacrylated collagen, hyaluronic acid, and gelatin (GelMA) hydrogels can be crosslinked with light and photoinitiators (Irgacure/LAP/Ruthenium), used as 3D cell culture scaffolds and bioinks for bioprinting.

Hydrogela are the most widely used systems for 3D cell culture. Learn more about this technology (what are hydrogels? How to chose?)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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