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PhotoHA-IRG, Methacrylated Hyaluronic Acid Hydrogel Kit

Photocrosslinked hyaluronic acid hydrogel (CC326 Kit Component 1)

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lyophilized powder


cell analysis

General description

3D cell culture, including bioprinting, allows for the creation of more physiological cell models by allowing cells to simultaneously interact with integrins on all cell surfaces, resulting in the activation of specific signaling pathways not activated in traditional 2D cell culture methods. Hydrogels are water swollen polymers that allow for the culture of cells in 3-dimensions and can have profound effects on cellular development, differentiation, migration, and function. New areas of tissue engineering such as 3D bioprinting, have utilized UV photocrosslinked methacrylated hydrogel biomaterials (PEGMA, GelMA, HAMA and ColMA etc.) to encapsulate cells to make printable bioinks. Hyaluronic acid is the most abundant glycosaminoglycan in the body being an important component of several tissues throughout the body. While it is abundant in extracellular matrices, hyaluronan also contributes to tissue hydrodynamics, movement and proliferation of cells, and participates in a number of cell surface receptor interactions. Hyaluronic acid is a polymer of disaccharides, themselves composed of D-glucuronic acid and Nacetyl- D-glucosamine, linked via alternating β-(1→4) and β-(1→3) glycosidic bonds. The PhotoHA-IRG, Methacrylated Hyaluronic Acid Hydrogel Kit (CC326: CC326-1, CC326-2) is based upon purified hyaluronic acid methacrylate (HAMA), which when photocrosslinked provides a native-like 3D environment for cells. In addition to hyaluronic acid methacrylate, the kit includes the photoinitiator Irgacure 2959 for users to easily fine tune their photocrosslinking experiments (i.e. altering hydrogel stiffness or gelling speeds).PhotoHA is a registered trademark of Advanced BioMatrix, Inc.


Photocrosslinking is initiated using a 365 nm UV light source.


1 Kit


CC326-1 (Store at 2-8°C): Irgacure Photoinitiator, 1 X 100 mg (CS226447).
Note: The complete PhotoHA-IRG, Methacrylated Hyaluronic Acid Hydrogel Kit (CC326) consists of CC326-1 and CC326-2 sold seperately.


Appearance: Lyophilized PowderSterility (USP modified): No GrowthGrafting Efficiency: 50-70%pH: 6.0-8.0Osmolality: 200-400 mOsmo H20/kg in 1X PBSMolecular Weight: 100 – 150 kDaNMR: CharacteristicCell Compatibility: Characteristic


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.




Hazard Classifications

Aquatic Chronic 2

Storage Class

11 - Combustible Solids



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