ECM555
QCM ECMatrix Cell Invasion Assay, 96-well (8 µm), fluorimetric
The CHEMICON Cell Invasion Assay Kit employs a 96-well plate, and provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.
Synonym(s):
8 μm pore cell invasion assay
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About This Item
UNSPSC Code:
12352207
eCl@ss:
32161000
NACRES:
NA.32
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Quality Level
species reactivity
human
manufacturer/tradename
Chemicon®
QCM
technique(s)
activity assay: suitable
cell based assay: suitable
detection method
fluorometric
shipped in
wet ice
Related Categories
General description
QCM ECMatrix Cell Invasion Assay involves invaded cells at the bottom of the insert membrane and can be obtained by treating the membrane with cell detachment buffer. The cells are lysed and detected by the patented CyQuant GR dye. This dye is a green-fluorescent dye and binds well with cellular nucleic acids to emit a strong fluorescence. This assay kit provides an efficient system for evaluating the invasion of tumor cells through a basement membrane model.
Application
QCM ECMatrix Cell Invasion Assay, 96-well (8 μm), fluorimetric has been used in the cell invasion assay of:
- human cervical carcinoma
- osteosarcoma
- breast cancer cells
- colorectal cancer cells
Features and Benefits
- The assay does not require cell labeling, scraping, washing, or counting
- Suitable for large-scale screening and quantitative comparison of multiple samples due to homogenous fluorescence detection and 96 well insert
- Enables a quick and efficient system for the quantitative determination of several factors for cell invasion
Components
Sterile 96-well Cell Invasion Plate Assembly: (Part No. 90200) One 96-well feeder tray, and one 96-well Cell Invasion Chamber plate with ECMatrixTM -coated inserts.
96-well Cell Culture Tray: (Part No. 90129) One 96-well feeder tray.
Cell Detachment Solution: (Part No. 90131) One bottle - 16 mL.
4X Cell Lysis Buffer: (Part No. 90130) One bottle - 16 mL.
CyQuant GR Dye: (Part No. 90132) One vial - 75 μL
96-well Cell Culture Tray: (Part No. 90129) One 96-well feeder tray.
Cell Detachment Solution: (Part No. 90131) One bottle - 16 mL.
4X Cell Lysis Buffer: (Part No. 90130) One bottle - 16 mL.
CyQuant GR Dye: (Part No. 90132) One vial - 75 μL
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1
Storage Class
10 - Combustible liquids
Certificates of Analysis (COA)
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