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MILLIPLEX® RTK Phosphoprotein Magnetic Bead Panel- Cell Signaling Multiplex Assay

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.

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eCl@ss:
32161000

Quality Level

species reactivity

human

manufacturer/tradename

Milliplex®

technique(s)

multiplexing: suitable

detection method

fluorometric (Luminex xMAP)

shipped in

ambient

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48-690MAG48-611MAG48-622MAG
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

shipped in

ambient

shipped in

-

shipped in

-

shipped in

-

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex xMAP)

detection method

fluorometric (Luminex® xMAP®)

detection method

fluorometric (Luminex® xMAP®)

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

General description

RTKs (Receptor Tyrosine Kinases) are transmembrane proteins that act as receptors for growth factors, neurotrophic factors and other extracellular signaling molecules. Upon binding extracellular ligands, RTKs can undergo receptor dimerization and tyrosine phosphorylation on specific residues in the cytoplasmic tails which then lead to RTK-mediated signal transduction. Of the greater than 500 known protein kinases in human genome, approximately 60 are members of the RTK family. They are central components of cell signaling networks and play crucial roles in normal physiological processes and disease processes ranging from diabetes to cancer.

The MILLIPLEX® RTK Phosphoprotein Bead Customizable Panel is used to detect changes in phosphorylated (panTyr) c-Kit, c-Met/HGFR, EGFR, ErbB2/HER2, ErbB3/HER3 , ErbB4/HER4 , FGFR1 , FLT3 , IGF-1R, M-CSFR, PDGFRα, PDGFRβ, TIE1, TIE2, VEGFR1, VEGFR2, and VEGFR3 in cell lysates using the Luminex® system.

The MILLIPLEX® portfolio offers the broadest selection of analytes across a wide range of pathways. Once the panel of interest has been identified, you can rely on the quality we build into each kit to produce results you can trust. Performance criteria evaluated during the validation process include: cross-reactivity, assay CVs, kit stability, and sample behavior. In addition, each kit meets stringent Quality Control criteria to ensure lot-to-lot reproducibility.

The MILLIPLEX® RTK Phosphoprotein Bead Panel includes:


  • Positive and negative control cell lysates provided to qualify assay performance
  • Configurable beads to capture analytes of interest
  • Optimized detection antibody cocktail designed to yield consistent analyte profiles within a panel


Panel Type: Cell Signaling

Specificity

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

  • Analytes: c-Kit, c-Met/HGFR (panTyr), EGFR (pan Tyr), ErbB2/HER2, ErbB3/HER3 (panTyr) ,ErbB4/HER4 (panTyr), FLT3 (pan Tyr), IGF-1R (pan Tyr), M-CSFR (pan Tyr), PDGFR-α (pan Tyr), PDGFRß (pan Tyr), TIE1 (pan Tyr,) Tie2 (pan Tyr), VEGFR1 (pan Tyr), VEGFR2 (pan Tyr), VEGFR3 (pan Tyr), FGFR1
  • This 17-plex panel, unlike most signaling kits that are fixed/premixed, is customizable so that it is possible to choose only those analytes that are wanted or needed.
  • Recommended Sample type: cell lysate
  • Recommended starting concentration: 40 to 1,000 μg protein/mL
  • Assay Run Time: Overnight
  • Research Category: Signaling Apoptosis & Cancer

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Packaging

96-well plate

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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Adam Stewart et al.
Molecular cancer therapeutics, 18(8), 1396-1404 (2019-07-03)
It is increasingly appreciated that drug response to different cancers driven by the same oncogene is different and may relate to differences in rewiring of signal transduction. We aimed to study differences in dynamic signaling changes within mutant KRAS (KRAS
Elizabeth A Coker et al.
Molecular cancer therapeutics, 21(6), 1020-1029 (2022-04-04)
We hypothesize that the study of acute protein perturbation in signal transduction by targeted anticancer drugs can predict drug sensitivity of these agents used as single agents and rational combination therapy. We assayed dynamic changes in 52 phosphoproteins caused by
Ahmet Acar et al.
Nature communications, 11(1), 1923-1923 (2020-04-23)
Drug resistance mediated by clonal evolution is arguably the biggest problem in cancer therapy today. However, evolving resistance to one drug may come at a cost of decreased fecundity or increased sensitivity to another drug. These evolutionary trade-offs can be

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Learn how multiplexing with MILLIPLEX® multiplex assays can help simplify cancer research by measuring multiple circulating biomarkers at once. Find multiplex panels to analyze JAK-STAT, mTOR, MAPK, SAPK, TGFβ signaling pathways, and more.

Uncover how cells communicate with MILLIPLEX® cell signaling multiplex assays. Multiplexing with cell signaling phosphoprotein assays based on Luminex® xMAP® technology helps researchers measure phosphoproteins and total proteins within the same or different pathways from a single sample.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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