M00PMCB24

m-ColiBlue24 Broth

ready-to-use, ampule of 2 mL, suitable for microbiology, for Escherichia spp., for coliforms

• Synonym(s): EC Broth, Coliform Broth
• UNSPSC Code: 41106225
• eCl@ss: 32160801
• NACRES: NA.74

Properties

• form: liquid
• Quality Level: 100
• shelf life: 12 mo.
• feature: ready-to-use
• packaging: ampule of 2 mL; pack of 50 tests
• color: blue
• input: sample type sport drink; sample type cider; sample type beer; sample type cosmetics; sample type food(s); sample type wine; sample type bottled water; sample type beverage(s); sample type soft drink
• pH: 7.0±0.2 (25.0 °C)
• application(s): bioburden testing; clinical; cosmetics; environmental; food and beverages; industrial qc; water monitoring; microbiology
• compatibility: for use with MF-method; for use with Microfil^®; for use with MicropreSure^®; for use with Milliflex^®
• shipped in: wet ice
• storage temp.: 2-8°C
• suitability: Escherichia spp.; coliforms

Description

General description

m-ColiBlue24^® broth is recommended for simultaneous detection of total coliform bacteria and Escherichia coli (E. coli) in water samples within 24 hours. An enzymatic indicator in the medium causes non-fecal total coliform colonies grown on the m-ColiBlue24^® medium to be red, while the E. coli (fecal coliform) colonies are blue. The selectivity of the enzymatic indicator eliminates the need for confirmation. The low false positive and false negative rates allow for the detection of at least 95% of all E. coli. m-ColiBlue24^® Broth enhances the growth rate of coliform bacteria. Special inhibitors in the medium efficiently minimize the growth of non-coliform bacteria.

Application

m-ColiBlue24^® broth is used for monitoring drinking water, wastewater, and other types of water using a 24 hour incubation period. It can also be used to detect coliforms in other types of water—bottled, surface, ground, well, recreational (fresh or marine), as well as process water for ultrapure chemical processing.

Features and Benefits

• Ready-to-use Liquid ampoule media solve numerous problems in microbiology laboratories where time, equipment, and trained personnel are often in short supply.
• They are available in convenient, single-test 2 mL plastic ampoules, eliminating the manipulation of glass ampoules and avoiding the waste and spoilage associated with bulk packaged media.

Specifications

EPA Approved

Incubation Temperature: 35 °C ± 0.5

Incubation Time: 24 hrs

Analysis Note

E.coli forms blue colonies. Other coliforms will show as red colonies. All other colors are non-coliform bacteria.

QC organisms
E. cloacae (ATCC 23355)

E. coli (ATCC 25922)

K. pneumoniae (ATCC 13883)

P. aeruginosa (ATCC 27853)

Target Microorganisms: Total coliforms and E. Coli

Legal Information

MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany

MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany

MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

m-ColiBlue24 is a registered trademark of Hach Company

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable