MHA000P2E

m-Endo Total Coliform Broth

ready-to-use, ampule of 2 mL, suitable for microbiology, for coliforms

• Synonym(s): MF Endo Medium, m-Coliform Broth
• UNSPSC Code: 41106225
• eCl@ss: 32160801
• NACRES: NA.74

Properties

• form: liquid
• Quality Level: 100
• shelf life: 12 mo.
• feature: ready-to-use
• packaging: ampule of 2 mL; pack of 50 tests
• color: pink to light purple, with slight precipitate
• input: sample type wine; sample type cosmetics; sample type food(s); sample type beverage(s); sample type cider; sample type sport drink; sample type bottled water; sample type soft drink; sample type beer
• pH: 7.2±0.2 (25.0 °C)
• application(s): bioburden testing; clinical; cosmetics; environmental; food and beverages; industrial qc; water monitoring; microbiology
• compatibility: for use with EZ-Fit^® Filtration Manifold; for use with MF-method; for use with MicropreSure^®; for use with Milliflex^®
• shipped in: wet ice
• storage temp.: 2-8°C
• suitability: Escherichia spp.; coliforms

Description

General description

m-Endo Total Coliform Broth is a selective and differential medium used to estimate the coliform bacteria in water samples by membrane filtration technique. The medium enables differentiation between lactose fermenters and non-fermenters. The use of sodium sulfite and basic fuchsin instead of bile salts inhibits the growth of Gram-positive bacteria. Lactose fermenting bacteria produce acetaldehyde that reacts with the sodium sulfite and fuchsin to form red colonies. The rapid fermentation of lactose by the organisms produces aldehydes, which leads to the development of a metallic sheen. Lactose non-fermenters form colorless colonies.

Application

m-Endo Total Coliform Broth is suitable for Total coliform detection in water samples. The media enables to differentiate between lactose fermenters from lactose non-fermenters.

Features and Benefits

• Ready-to-use Liquid ampoule media solve numerous problems in microbiology laboratories where time, equipment, and trained personnel are often in short supply.
• They are available in convenient, single-test 2 mL plastic ampoules, eliminating the manipulation of glass ampoules and avoiding the waste and spoilage associated with bulk packaged media.
  

Specifications

Incubation Temperature: 35 °C ± 0.5

Incubation Time: 24 hrs ± 2

Standard Methods for the Examination of Water and Wastewater

Analysis Note

Coliform colonies appear deep red with distinct green metallic sheen

QC organisms
E. coli (ATCC 25922)

Mixed culture (E. coli, P. aeruginosa and P. vulgaris)

Target Microorganisms: Total coliforms

Legal Information

EZ-FIT is a registered trademark of Merck KGaA, Darmstadt, Germany

MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany

MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable