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MHA00P2TT

Millipore

Tryptone Glucose Extract Broth with TTC

ampule of 2 mL, ready-to-use, suitable for microbiology, for heterotrophic bacteria

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Synonym(s):
TGE broth with TTC, TGE broth with triphenyl tetrazolium chloride
eCl@ss:
32160801
NACRES:
NA.74

form

liquid

Quality Level

shelf life

12 mo.

feature

ready-to-use

packaging

ampule of 2 mL
pack of 50 tests

technique(s)

cell counting: suitable (total viable count (TVC))

color

clear medium amberslightly opalescent

input

sample type beverage(s)
sample type food(s)
sample type cider
sample type bottled water
sample type beverage(s)
sample type beer
sample type cosmetics
sample type wine
sample type sport drink
sample type soft drink

pH

7.0±0.2 (25.0 °C)

application(s)

bioburden testing
clinical
cosmetics
environmental
food and beverages
industrial qc (microbial monitoring)
water monitoring

microbiology

compatibility

for use with MF-method
for use with Microfil®
for use with MicropreSure®
for use with Milliflex®

shipped in

wet ice

storage temp.

2-8°C

suitability

heterotrophic bacteria
nonselective and differential for

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technique(s)

cell counting: suitable (total viable count (TVC))

technique(s)

-

technique(s)

-

technique(s)

microbiological culture: suitable

suitability

heterotrophic bacteria, nonselective and differential for

suitability

selective and differential for Citrobacter spp., selective and differential for Enterobacter spp., selective and differential for Escherichia coli, selective and differential for Proteus spp., selective and differential for Salmonella spp., selective and differential for Shigella spp., selective and differential for coliforms

suitability

nonselective for

suitability

selective for Escherichia coli, selective for coliforms, selective for enterovirulent E. coli (EEC)

application(s)

bioburden testing
clinical
cosmetics
environmental
food and beverages
industrial qc (microbial monitoring)
water monitoring, microbiology

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring, microbiology

application(s)

pharmaceutical
sterility testing

application(s)

agriculture
food and beverages
microbiology
pathogen testing

pH

7.0±0.2 (25.0 °C)

pH

-

pH

7.3

pH

-

Quality Level

100

Quality Level

200

Quality Level

400

Quality Level

200

General description

Tryptone Glucose Extract Broth with TTC is recommended for the enumeration of bacteria in water, air, milk and dairy products. It is used as a standard medium for the bacteriological plate count and is also used for the detection of thermophilic bacteria in milk and dairy products. Tryptone and yeast extract provide nitrogenous and carbonaceous compounds, long-chain amino acids, vitamin B complex and other essential growth nutrients. Glucose is the energy source, TTC is used as an indicator for easy enumeration. Colonies appear red. Red colour in the colonies is produced by reduction of triphenyl tetrazolium chloride (TTC).

Application

Tryptone Glucose Extract Broth with TTC is used as a non-selective medium to detect total heterotrophic microorganisms in water, dairy products and other liquids like Beer, Bottled Water, Cider, Soft Drink, Sport Drink and Wine. The media finds its application in Clinical, Cosmetics, Environmental Analysis, Food and Beverage and Industrial QC.

Features and Benefits

  • Ready-to-use Liquid ampoule media solve numerous problems in microbiology laboratories where time, equipment, and trained personnel are often in short supply.
  • They are available in convenient, single-test 2 mL plastic ampoules, eliminating the manipulation of glass ampoules and avoiding the waste and spoilage associated with bulk packaged media.

Specifications

Incubation Temperature: 25–35 °C
Incubation Time: 24–72 hrs

Analysis Note

Colonies appear red. Red color in the colonies is produced by reduction of triphenyl tetrazolium chloride (TTC)
QC organisms
E. coli (ATCC 25922)
Target Microorganisms: Total Count

Legal Information

MICROFIL is a registered trademark of Merck KGaA, Darmstadt, Germany
MICROPRESURE is a registered trademark of Merck KGaA, Darmstadt, Germany
MILLIFLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Compendium of methods for the microbiological examination of foods. 2001
American Public Health Association and others
American Public Health Association (2001)

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