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MAB1596

Sigma-Aldrich

Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9

clone 6G6-1C9, Chemicon®, from mouse

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Synonym(s):
PSD-95
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6G6-1C9, monoclonal

species reactivity

mouse, rat, bovine

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG2a

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

bovine ... Dlg4(100137840)
mouse ... Dlg4(13385)
rat ... Dlg4(29495)

General description

PSD-95 is part of a multiprotein complex restricted to the post-synaptic membrane. It is believed to initiate NMDA receptor clustering on the post synaptic membrane. Its restricted expression makes it an excellent marker for post-synaptic membrane regions on neurons.

Specificity

Other species have not been tested.
Reacts with a protein of ~100 kDa, corresponding to the apparent molecular weight of PSD-95, on SDS-PAGE immunoblots of samples from rat, mouse and bovine. A slightly higher molecular weight band is also detected. In rat brain and mouse brain tissue extracts a band of ~75 kDa is also present.

Immunogen

Recombinant rat PSD-95.

Application

Anti-Post Synaptic Density Protein 95 Antibody, clone 6G6-1C9 detects level of Post Synaptic Density Protein 95 & has been published & validated for use in IC, IH & WB.
Immunocytochemistry:
Representative image from a previous lot.
Confocal fluorescent analysis of PC12 using MAb1596 Mouse mAB (Red). Actin filaments have been labeled with Alexa Fluor 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue). Membrane staining positive.

Immunocytochemistry:
12.5 µg/mL

Western Blot:
1-5 μg/mL (ECL) on rat brain lysate (20 μg).

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Synapse & Synaptic Biology

Quality

Routinely evaluated in Immunocytochemistry.

Target description

~100 kDa

Linkage

Replaces: 04-1066

Physical form

Format: Purified
Purified immunoglobulin. Liquid in PBS, pH 7.2 with 50% glycerol and 0.09% sodium azide.
See datasheet for purification method.

Storage and Stability

Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Do not store below -20°C.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Control
Mouse brain, rat brain tissue.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


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