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Key Documents

MAB1912

Sigma-Aldrich

Anti-Collagen Type I (COL1A1) Antibody

CHEMICON®, rat monoclonal, M-58

Synonym(s):

Anti-Procollagen Antibody, Clone M-58 Anti-Procollagen, Type I Procollagen Detection

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Pricing and availability is not currently available.

Product Name

Anti-Procollagen Type I Antibody, NT, clone M-58, culture supernatant, clone M-58, Chemicon®

biological source

rat

Quality Level

antibody form

culture supernatant

clone

M-58, monoclonal

species reactivity

human

should not react with

rat, hamster, mouse

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable (paraffin)

isotype

IgG2α

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Show Differences

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This Item
AB758AB745MAB3391
clone

M-58, monoclonal

clone

polyclonal

clone

polyclonal

clone

5D8-G9, monoclonal

antibody form

culture supernatant

antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin (Semi-purified)

antibody form

purified immunoglobulin

species reactivity

human

species reactivity

human, bovine

species reactivity

human

species reactivity

pig, canine, sheep, human, bovine

biological source

rat

biological source

goat

biological source

rabbit

biological source

mouse

technique(s)

immunohistochemistry: suitable (paraffin)

technique(s)

ELISA: suitable, dot blot: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: suitable, flow cytometry: suitable, immunohistochemistry: suitable, western blot: suitable

Specificity

Recognizes Human pro-collagen I. MAB1912 labels the amino-terminal pro-peptide.

Application

Detect Procollagen Type I using this Anti-Procollagen Type I Antibody, N-terminus, clone M-58 validated for use in IH(P).
Immunohistochemistry: 1:10,000-1:50,000 (fresh frozen tissue sections.)

Suitable for formaldehyde/formalin fixed paraffin sections, suggested starting dilution 1:100-1:1000, after treatment with 1% trypsin, 20 minutes at room temperature. Does not stain mature collagen fibers in tissue. Localized intracellularly in cells producing pro-collagen I. Not recommended for Western Blots Optimal working dilutions must be determined by end user.

Analysis Note

Control
Skin tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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