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SAB4500362

Sigma-Aldrich

Anti-Collagen I antibody produced in rabbit

affinity isolated antibody

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Synonym(s):
α 2 type i collagen, co1a2, col1a2, collagen, type i
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 129 kDa

species reactivity

mouse, human, rat

concentration

~1 mg/mL

technique(s)

ELISA: 1:5000
immunofluorescence: 1:100-1:500
immunohistochemistry: 1:50-1:100

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... COL1A2(1278)

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This Item
SAB5700818SAB4500366SAB4500388
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

mol wt

antigen 129 kDa

mol wt

129

mol wt

antigen 141 kDa

mol wt

antigen 108 kDa

UniProt accession no.

P08123

UniProt accession no.

P08123

UniProt accession no.

P02458

UniProt accession no.

P12110

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General description

Collagen I is encoded by the gene COL1A2 (collagen type I alpha 2). The gene is located on human chromosome 7q21.3. COL1A2 is a fibril forming collagen, found in bone, dermis, tendon, ligaments and cornea.
Collagen is the major structural component of extracellular matrices found in connective tissues and internal organs, but is most prevalent in the dermis, tendons, and bones. It is classified into a number of structurally and genetically distinct types. Type I collagen is a heterodimer composed of two α1(I) chains and one α2(I) chain that spontaneously forms a triple helix scaffold at neutral pH and 37 °C. Collagen type I is an excellent substrate for the culture of hepatocytes, fibroblasts, spinal ganglion, muscle cells, Schwann cells, embryonic lung cells, epithelial cells, and a number of other cell lines. It has also been used in the study of growth, differentiation, migration of cell lines, and tissue morphogenesis during development.
Rabbit polyclonal anti-Collagen I, N-Terminal antibody reacts with mouse, rat, and human type I collagens.

Immunogen

The antiserum was produced against synthesized peptide derived from human Collagen I.

Immunogen Range: 1-50

Application

Anti-Collagen I antibody produced in rabbit has been used in western blotting and immunostaining.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Rabbit polyclonal anti-Collagen I, N-Terminal antibody is used to tag collagen type I for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as ELISA, immunoblotting, and immunoprecipitation. It is used as a probe to determine the presence and roles of collagen type I as a cell substrate.

Biochem/physiol Actions

Collagen I interacts with cytoskeleton and regulates cell motility. Mutations in this gene is linked to osteoporotic fracture, osteogenesis imperfecta and recessive classical type Ehlers-Danlos syndrome. It is also associated with idiopathic osteoporosis and atypical Marfan syndrome.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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The importance of polymorphic variants of collagen 1A2 gene (COL1A2) in the development of osteopenia and osteoporosis in postmenopausal women
Majchrzycki M, et al.
Ginekologia Polska, 88(8), 414-420 (2017)
Joana C Antunes et al.
Tissue engineering. Part A, 21(11-12), 1869-1885 (2015-03-12)
Cartilage damage and/or aging effects can cause constant pain, which limits the patient's quality of life. Although different strategies have been proposed to enhance the limited regenerative capacity of cartilage tissue, the full production of native and functional cartilaginous extracellular
Collagenase 1A2 (COL 1A2) gene A/C polymorphism in relation to severity of dental fluorosis
Escobar-Garcia D, et al.
Community dentistry and oral epidemiology, 44(2), 162-168 (2016)
Long-term characterization of axon regeneration and matrix changes using multiple channel bridges for spinal cord regeneration
Tuinstra HM, et al.
Tissue Engineering: Part A, 20(5-6), 1027-1037 (2013)
Nastaran Tarban et al.
Cells, 11(8) (2022-04-24)
Skeletal muscle repair is initiated by local inflammation and involves the engulfment of dead cells (efferocytosis) by infiltrating macrophages at the injury site. Macrophages orchestrate the whole repair program, and efferocytosis is a key event not only for cell clearance

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