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MAB3152

Sigma-Aldrich

Anti-Cardiac Troponin I Antibody, a.a. 87-91, clone 8E10

clone 8E10, Chemicon®, from mouse

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Synonym(s):
cTn1, Cardiac Troponin I
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

clone

8E10, monoclonal

species reactivity

canine, rabbit, feline, human, bovine, pig, goat

should not react with

rat, mouse, fish

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Specificity

MAB3152 reacts equally with free cardiac troponin 1 (cTn1) and cTn1 forming complexes with other troponin components. It recognizes epitopes that are not perturbed or sterically shielded by other troponin components. Not affected by heparin, phosphorylation, oxidation and complex formation. Recognizes an epitope located between amino acid residues 87 and 91 of cTnI. There is no cross-reaction with skeletal muscle troponin 1.

Immunogen

Epitope: a.a. 87-91
Free human cardiac troponin 1.

Application

Detect Cardiac Troponin I using this Anti-Cardiac Troponin I Antibody, a.a. 87-91, clone 8E10 validated for use in ELISA & WB.
Research Category
Metabolism
Research Sub Category
Muscle Physiology
Western blotting: identifies two bands 14kDa and 18kDa which appear to be breakdown products of full length cTn1 (Morjana N. 1998, Degradation of human cardiac troponin I after myocardial infarction. Biotechnol Appl. Biochem. 28:105-11).

EIA: MAB3152 has been used successfully in ELISA and immunoaffinity purification. Its affinity constant is 8x10(10). It can be used with MAB3150 in sandwhich ELISA as the coating antibody.

Optimal working dilutions must be determined by end user.

Physical form

Format: Purified
Liquid in PBS (pH 7.4) with 0.1% sodium azide.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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