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MAB3199Z-C

Sigma-Aldrich

Anti-E-Cadherin Antibody, clone 67A4, Azide & Ascites Free

clone 67A4, 0.5 mg/mL, from mouse

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Synonym(s):
Cadherin-1, CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD324
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

67A4, monoclonal

species reactivity

human

concentration

0.5 mg/mL

technique(s)

flow cytometry: suitable
immunofluorescence: suitable
inhibition assay: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CDH1(999)

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This Item
05-915MABT1579MABT26
clone

67A4, monoclonal

clone

13A9, monoclonal

clone

2B6-D8, monoclonal

clone

Decma-1, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rat

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human, mouse

antibody form

purified antibody

antibody form

culture supernatant

antibody form

purified antibody

antibody form

purified antibody

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

100

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General description

Cadherins are a family of transmembrane glycoproteins involved in Ca2+-dependent cell to cell interactions. The members of the family are differentially expressed in various tissues and function in the maintenance of tissue integrity, morphogenesis, and migration. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin), neural cadherin (N-cadherin), placental cadherin (P-cadherin) and retinal cadherin (R-cadherin). Type II include kidney cadherin (K-cadherin) and osteoblast cadherin (OB-cadherin). One of the best characterized cadherins is E-cadherin (epithelial cadherin or cadherin-1). It consists of calcium binding extracellular domains responsible for interactions with other E-cadherin molecules on neighboring cells and interaction with integrin alphaEbeta7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through cytoplasmic catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas E-cadherin is frequently downregulated, which is consistent with its function of an invasion suppressor in normal epithelia.

Immunogen

Whole cells corresponding to human E-Cadherin.

Application

Flow Cytometry Analysis: 2 µg from a representative lot detected E-Cadherin in 1X10E6 A431 cells.

Flow Cytometry Analysis: A representative lot from an independent laboratory detected E-Cadherin in bone marrow cells (Burhing, H. J., et al. (1996). Leukemia. 10(1):106-116.)

Inhibition Analysis: A representative lot from an independent laboratory inhibited E-Cadherin-mediated adhesion of Langerhans to keratinocytes (Caberg, J. H., et al. (2008). Carcinogenesis. 29(7):1441-1447.).

Inhibition Analysis: A representative lot from an independent laboratory inhibits human embryoid body agglomeration (Dang, S. M., et al. (2004). Stem Cells. 22: 275-282.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected E-Cadherin in human thymus tissue (Kutlesa, S., et al. (2002). J Cell Sci. 115(Pt. 23):4505-4515.).

Western Blotting Analysis: A representative lot from an independent laboratory detected E-Cadherin in human thymus tissue lysate (Kutlesa, S., et al. (2002). J Cell Sci. 115(Pt. 23):4505-4515.).
Research Category
Cell Structure
Research Sub Category
ECM Proteins
This Anti-E-Cadherin Antibody, clone 67A4, Azide & Ascites Free is validated for use in western blotting, flow cytometry, inhibition & immunofluorescence for the detection of E-Cadherin.

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected E-Cadherin in 10 µg of A431 cell lysate.

Target description

~97 kDa observed

Linkage

Replaces: 04-1103

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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