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SAB4503751

Sigma-Aldrich

Anti-E-cadherin antibody produced in rabbit

affinity isolated antibody

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Synonym(s):
CAM 120/80, CD324, Cadherin-1, Epithelial cadherin, Uvomorulin
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 97 kDa

species reactivity

human

concentration

~1 mg/mL

technique(s)

ELISA: 1:40000
immunofluorescence: 1:100-1:500
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CDH1(999)

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This Item
SAB4300693SAB5700789SAB5700696
conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

-

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

antigen 97 kDa

mol wt

~135 kDa

mol wt

90, 97

mol wt

90, 97

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General description

Anti-E-cadherin Antibody detects endogenous levels of total E-cadherin protein.
CDH1 (cadherin 1) codes for a transmembrane glycoprotein E-cadherin that is located on human chromosome 16q22.1. It is a tumor suppressor gene, which is also known as Cdh1p/Hct1p, Fizzy-related, Ste9, or Srw1.

Immunogen

The antiserum was produced against synthesized peptide derived from human E-cadherin.

Immunogen Range: 1-50

Application

Anti-E-cadherin, N-Terminal antibody has been used in protein extraction,western blotting, confocal fluorescence microscopy analysis, immunostaining and co-immunoprecipitation.

Biochem/physiol Actions

CDH1 (cadherin 1) helps to conserve the homophilic cell-cell adhesion in epithelial tissues. Inadequate expression of CDH1 results in colon cancer and renal cell carcinoma (RCC).

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Electric field exposure promotes epithelial?mesenchymal transition in human lens epithelial cells via integrin ?1?FAK signaling
Liu J, et al.
Molecular Medicine Reports, 16(4), 4008-4014 (2017)
TUSC3 promotes colorectal cancer progression and epithelial-mesenchymal transition (EMT) through WNT/?-catenin and MAPK signalling
Gu Y, et al.
The Journal of Pathology (2016)
Mitotic Regulation of the APC Activator Proteins CDC20 and CDH1
Kramer ER, et al.
Molecular Biology of the Cell (2000)
Junji Itou et al.
FEBS letters, 587(18), 3115-3121 (2013-08-21)
In cell cultures, the dispersed phenotype is indicative of the migratory ability. Here we characterized Sal-like 4 (SALL4) as a dispersion factor in basal-like breast cancer. Our shRNA-mediated SALL4 knockdown system and SALL4 overexpression system revealed that SALL4 suppresses the
CDH1 promoter hypermethylation and E-cadherin protein expression in infiltrating breast cancer
Caldeira JR, et al.
BMC Cancer (2006)

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