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V1514

Sigma-Aldrich

Anti-VE-Cadherin (CD144) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Anti-CD144, Anti-Cadherin-5, Anti-Vascular Endothelial Cadherin
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~130 kDa

species reactivity

human

technique(s)

immunohistochemistry (frozen sections): 1:100 using acetone-fixed sections of human tonsil
western blot: 1:500 using whole extract of human umbilical vein endothelial cells (HUVEC).

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CDH5(1003)

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This Item
SAB1306131SAB4504676MABT134
biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

mouse

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

conjugate

-

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

100

technique(s)

immunohistochemistry (frozen sections): 1:100 using acetone-fixed sections of human tonsil, western blot: 1:500 using whole extract of human umbilical vein endothelial cells (HUVEC).

technique(s)

flow cytometry: 1:10-1:50, western blot: 1:2000

technique(s)

ELISA: 1:20000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-

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General description

Cadherin 5 (CDH5), also known as cadherin 144 (CD144), is encoded by the gene mapped to long arm of human chromosome 16. The encoded protein is expressed in endothelial cells, but it absent in normal epithelium.
VE-Cadherin (also designated vascular endothelial cadherin, cadherin-5, and cluster of differentiation 144 (CD144)) is a non-type I or II cadherin that is expressed on endothelial cells of all blood and lymphatic vessels, where it organizes intercellular adherens junctions.

Immunogen

synthetic peptide corresponding to amino acids 91-110 of human VE-cadherin (CD144).

Application

Anti-VE-Cadherin (CD144) antibody produced in rabbit has been in following studies:
  • immunofluorescence imaging
  • western blotting
  • to weaken cell-cell junctions in human umbilical vein endothelial cells (HUVECs)
  • immunohistochemistry

Biochem/physiol Actions

Cadherin 5 (CDH5)/CD144 plays an essential role in various cellular processes, such as regulation of cell adhesion, cell proliferation, cell survival, cell shape, cell motility. Additionally, it is also involved in the regulation of signaling pathways and transcriptional gene regulation. CD144/VE-cadherin is a core component of endothelial adherens junction, which plays a vital role in angiogenic sprouting by enhancing cell migration and cell elongation. CD144 expressed in glioblastoma multiforme (GBM) facilitates the transdifferentiation of glioblastoma stem like cells (GSCs) to endothelial cells and form blood vessels, under hypoxia condition. VE-cadherin induces endothelial cell-cell adhesion at adherens junctions (AJs). CD144 gene knockout results in loss of cell polarity and modification in vascular lumenal structure. CD144 inhibits the synthesis of CDH2 (also known as N-cadherin) in the endothelium.
VE-cadherin (CD144) interacts with β-catenin, plakoglobinand Adherens junction protein (p120ctn).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Cellular and Molecular Bioengineering, 4, 9-27 (2011)
Localization of human cadherin genes to chromosome regions exhibiting cancer-related loss of heterozygosity.
Kremmidiotis G
Genomics, 49, 467-471 (1998)
Regulation of endothelial barrier function and growth by VE-cadherin, plakoglobin, and
Venkiteswaran K, et al.
American Journal of Physiology. Cell Physiology (2002)
Functionally specialized junctions between endothelial cells of lymphatic vessels
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The Journal of Experimental Medicine, 204(10), 2349-2349 (2007)

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