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MABT886

Sigma-Aldrich

Anti-VE-Cadherin Antibody, clone Vli37

culture supernatant, clone Vli37, from rabbit

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Synonym(s):
Cadherin-5, CD144, Vascular endothelial cadherin, VE-Cad, VE-cadherin, VEcad
eCl@ss:
32160702

biological source

rabbit

Quality Level

antibody form

culture supernatant

antibody product type

primary antibodies

clone

Vli37, monoclonal

species reactivity

bovine, mouse

species reactivity (predicted by homology)

rat (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

mouse ... Cdh5(12562)

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C1821MABF3160MABT134
antibody form

culture supernatant

antibody form

ascites fluid

antibody form

purified antibody

antibody form

purified immunoglobulin

clone

Vli37, monoclonal

clone

CH-19, monoclonal

clone

8C11, monoclonal

clone

BV6, monoclonal

species reactivity

bovine, mouse

species reactivity

canine, frog, snake, guinea pig, chicken, pig, feline, bovine, rabbit, hamster, Psammomys (sand rat), goat, rat, human, sheep, mouse

species reactivity

hamster, human

species reactivity

human

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested animal heart sections, indirect immunofluorescence: 1:500 using cultured MDBK cells, microarray: suitable, western blot: suitable

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, inhibition assay: suitable, western blot: suitable

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

isotype

IgG

isotype

IgG1

isotype

IgG1κ

isotype

IgG2aκ

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General description

Cadherin-5 (UniProt P55284; also known as Vascular endothelial cadherin, VE-Cad, VE-cadherin, VEcad, CD144) is encoded by the Cdh5 gene (Gene ID 12562) in murine species. Cadherins (calcium-dependent adhesion) are type-1 transmembrane proteins that form adherens junctions in tissues and play important roles in mediating cell adhesion. Cadherins are designated with a prefix that specifies their tissue association. Vascular endothelial-cadherin (VE-cadherin) is the transmembrane component of the endothelial adherens junction between vascular endothelial cells (ECs) and plays a pivotal role in endothelium integrity and in the control of vascular permeability. One characteristic of VEGF-induced vascular permeability is the phosphorylation of VE-cadherin, which leads to VE-cadherin internalization and the destabilization of adherens junctions. Likewise, VE-cadherin overexpression is shown to decrease the permeability of endothelial monolayers in vitro. VE-cadherin is initially produced with a signal peptide (a.a. 1-24) and a propeptide (a.a. 25-45) sequence, the removal of which yields tthe mature protein containing a large extracellular region (a.a. 46-599) with five cadherine repeats (a.a. 46-149, 150-256, 257-371, 372-476, 477-593), followed by a transmembrane segment (a.a. 600-620) and a cytoplasmic domain (a.a. 621-784).

Specificity

Clone Vli37 stains endothelial adherens junctions by immunocytochemistry and immunohistochemistry.

Immunogen

Epitope: Near C-terminus.
Linear peptide corresponding to the C-terminal cytoplasmic domain sequence of mouse VE-Cadherin.

Application

Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)
This Anti-VE-Cadherin Antibody, clone Vli37 is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Immunocytochemistry for the detection of VE-Cadherin.
Western Blotting Analysis: An 1:500 dilution of this antibody detected VE-Cadherin in 10 µg of mouse brain endothelial bEnd.3 cell lysate.
Western Blotting Analysis: An 1:2,000 dilution from a representative lot detected VE-cadherin expression in lysastes from mouse lung tissue, mouse brain endothelial bEnd.3 cells, and bovine aortic endothelial GM7372 cells (Courtesy of Dr. Volkhard Lindner, Maine Medical Center Research Institute, Scarborough, ME).
Immunohistochemistry Analysis: An 1:250-5,000 dilution from a representative lot detected VE-cadherin immunoreactivity in formalin-fixed, paraffin-embeded mouse lung, aorta, and liver tissue sections (Courtesy of Dr. Volkhard Lindner, Maine Medical Center Research Institute, Scarborough, ME).
Immunocytochemistry Analysis: An 1:500-5,000 dilution from a representative lot detected VE-cadherin immunoreactivity in bovine aortic endothelial GM7372 cells (Courtesy of Dr. Volkhard Lindner, Maine Medical Center Research Institute, Scarborough, ME).

Quality

Evaluated by Western Blotting in mouse lung tissue lysate.

Western Blotting Analysis: An 1:500 dilution of this antibody detected VE-Cadherin in 10 µg of mouse lung tissue lysate.

Target description

~110 observed. Target band size appears larger than the calculated molecular weight of 83.05 kDa due to glycosylation.

Physical form

Rabbit monoclonal IgG in supernatant with 0.05% sodium azide.
Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


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Chi Zhang et al.
Arteriosclerosis, thrombosis, and vascular biology, 38(11), 2691-2705 (2018-10-26)
Objective- Blood-CNS (central nervous system) barrier defects are implicated in retinopathies, neurodegenerative diseases, stroke, and epilepsy, yet, the pathological mechanisms downstream of barrier defects remain incompletely understood. Blood-retina barrier (BRB) formation and retinal angiogenesis require β-catenin signaling induced by the
Peter Baluk et al.
Cell and tissue research, 395(1), 81-103 (2023-11-30)
Endothelial cells of mammalian blood vessels have multiple levels of heterogeneity along the vascular tree and among different organs. Further heterogeneity results from blood flow turbulence and variations in shear stress. In the aorta, vascular endothelial protein tyrosine phosphatase (VE-PTP)
Keisuke Shirakura et al.
EMBO molecular medicine, 15(4), e16128-e16128 (2023-02-07)
Vascular endothelial protein tyrosine phosphatase (VE-PTP) influences endothelial barrier function by regulating the activation of tyrosine kinase receptor Tie2. We determined whether this action is linked to the development of atherosclerosis by examining the influence of arterial shear stress on

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