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MAB3303

Sigma-Aldrich

Anti-Collagen Type VI Antibody, clone VI-26

clone VI-26, Chemicon®, from mouse

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Synonym(s):
Anti-BTHLM1, Anti-OPLL, Anti-UCHMD1
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

VI-26, monoclonal

species reactivity

human, rabbit

should not react with

rat

manufacturer/tradename

Chemicon®

availability

not available in Japan

technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... COL6A1(1291)

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This Item
MAB2500SAB2100465MAB1330
biological source

mouse

biological source

mouse

biological source

rabbit

biological source

mouse

Gene Information

human ... COL6A1(1291)

Gene Information

human ... COL7A1(1294)

Gene Information

human ... COL6A1(1291)

Gene Information

human ... COL2A1(1280)

species reactivity

human, rabbit

species reactivity

bovine, human

species reactivity

dog, pig, rabbit, human

species reactivity

sheep, human, bovine, rat, pig, mouse, canine

clone

VI-26, monoclonal

clone

32, monoclonal, mAb-VII, monoclonal

clone

polyclonal

clone

COLL-II, monoclonal

Quality Level

100

Quality Level

100, 300

Quality Level

100

Quality Level

100

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Specificity

Specifically reacts with the hCL(VI). This is a purified mouse monoclonal antibody to human type VI collagen. VI-26 does not react with denatured or reduced type VI collagen, although this antibody does recognize the the triple helix consisting of alpha1(VI), alpha 2(VI) alpha3(gamma). The epitope has not been mapped and the monoclonal is know not to react with collagens I, II, III, IV or V in native assays.

Application

Anti-Collagen Type VI Antibody, clone VI-26 detects level of Collagen Type VI & has been published & validated for use in ELISA, IH(P).
Immunohistochemistry on acetone fixed paraffin-embedded tissues. Formalin fixation is not recommended. Antigen recovery is treatment with 0.04% trypsin in 0.01M CaCl(2) 0.05M Tris-HCL pH 7.6, 37°C for 10 minutes before the non-specific peroxidase block.

EIA

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Physical form

Format: Purified
Liquid in 0.1 M sodium phosphate buffer, pH 7.0, containing 2% protease-free bovine Serum albumin.
Protein A purified

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Testis, connective tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Tiziana Triulzi et al.
PloS one, 8(2), e56761-e56761 (2013-02-27)
We recently showed that differential expression of extracellular matrix (ECM) genes delineates four subgroups of breast carcinomas (ECM1, -2, -3- and -4) with different clinical outcome. To further investigate the characteristics of ECM signature and its impact on tumor progression
D O Visscher et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 107(5), 1711-1721 (2018-11-02)
The aim of this study was to design and manufacture an easily assembled cartilage implant model for auricular reconstruction. First, the printing accuracy and mechanical properties of 3D-printed poly-ε-caprolactone (PCL) scaffolds with varying porosities were determined to assess overall material
Laura K Zamurs et al.
The Journal of biological chemistry, 290(7), 4272-4281 (2014-12-24)
Bethlem myopathy and Ullrich congenital muscular dystrophy (UCMD) sit at opposite ends of a clinical spectrum caused by mutations in the extracellular matrix protein collagen VI. Bethlem myopathy is relatively mild, and patients remain ambulant in adulthood while many UCMD
Sara Aguti et al.
Methods in molecular biology (Clifton, N.J.), 2176, 221-230 (2020-09-01)
Allele-specific gene silencing by antisense oligonucleotide (ASO) or small interference RNA (siRNA) has been used as a therapeutic approach for conditions caused by dominant gain-of-function mutations. We here present an antisense approach using gapmer ASO to diminish the dominant-negative effect
Enrico Almici et al.
Frontiers in bioengineering and biotechnology, 10, 851825-851825 (2022-05-14)
Collagen VI-related dystrophies (COL6-RDs) are a group of rare congenital neuromuscular dystrophies that represent a continuum of overlapping clinical phenotypes that go from the milder Bethlem myopathy (BM) to the severe Ullrich congenital muscular dystrophy, for which there is no

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