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Key Documents

MAB333

Sigma-Aldrich

Anti-Synaptobrevin Antibody, clone SP10

ascites fluid, clone SP10, Chemicon®

Synonym(s):

VAMP

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

SP10, monoclonal

species reactivity

rat, hamster, salamander, pig, human

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgM

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... VAMP1(6843)

Specificity

Synaptobrevin [VAMP]. MAB333 reacts with VAMP-1 and VAMP-2 fusion protein expressed in bacterial system.

Immunogen

Crude synaptic immunoprecipitate (Human).

Application

Anti-Synaptobrevin Antibody, clone SP10 is an antibody against Synaptobrevin for use in WB, IH.
Immunohistochemistry at >1:25.

Western blotting at 1:100-1:10,000.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Synapse & Synaptic Biology

Linkage

Replaces: AB5863P

Physical form

Liquid, contains sodium azide.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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G Bonanno et al.
British journal of pharmacology, 129(8), 1780-1786 (2000-04-26)
The release of [(3)H]-dopamine ([(3)H]-DA) from human neocortex nerve terminals was studied in synaptosomes prepared from brain specimens removed in neurosurgery and exposed during superfusion to different releasing stimuli. Treatment with 15 mM KCl, 100 microM 4-aminopyridine, 1 microM ionomycin
Huayu Qi et al.
Molecular biology of the cell, 13(2), 530-541 (2002-02-21)
The zona pellucida (ZP) is a highly organized extracellular coat that surrounds all mammalian eggs. The mouse egg ZP is composed of three glycoproteins, called mZP1-3, that are synthesized, secreted, and assembled into a ZP exclusively by growing oocytes. Here
Elizabeth Scarr et al.
Clinical psychopharmacology and neuroscience : the official scientific journal of the Korean College of Neuropsychopharmacology, 10(1), 25-33 (2013-02-23)
Given the ability of mood stabilizers and antipsychotics to promote cell proliferation, we wanted to determine the effects of these drugs on neuronal markers previously reported to be altered in subjects with psychiatric disorders. Male Sprauge-Dawley rats were treated with
M Raiteri et al.
Journal of neurochemistry, 74(1), 423-431 (2000-01-05)
Small molecules present during brain tissue homogenization are known to be entrapped within subsequently isolated synaptosomes. We have revisited this technique in view of its systematic utilization to incorporate into nerve endings impermeant probes of large size. Rat neocortical synaptosomes
Richard Henriksson et al.
Synapse (New York, N.Y.), 62(11), 829-833 (2008-08-23)
Convergent lines of evidence suggest potentiation of glutamatergic synapses after chronic ethanol exposure, and indicate that the presynaptic effect hereof is on modulators of synaptic strength rather than on executors of glutamate release. To address this hypothesis in the context

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