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MAB343

Sigma-Aldrich

Anti-APP Antibody, APP 643-695 CT fragment, clone 2.F2.19B4

ascites fluid, clone 2.F2.19B4, Chemicon®

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Synonym(s):
APP, Jonas clone
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

clone

2.F2.19B4, monoclonal

species reactivity

rat, human

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... APP(351)

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This Item
AB5076MAB348AB5352
antibody form

ascites fluid

antibody form

serum

antibody form

purified immunoglobulin

antibody form

serum

clone

2.F2.19B4, monoclonal

clone

polyclonal

clone

22C11, monoclonal

clone

polyclonal

species reactivity

rat, human

species reactivity

human, mouse

species reactivity

rat, fish, human, mouse, canine, pig, monkey

species reactivity

human, monkey

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

Specificity

Reacts with intact full-length Alzheimer precursor protein (APP) and selectively with the cytoplasmic carboxyl fragment of APP 643-695. Epitope has reportedly been mapped in this paper http://www.impub.co.uk/abs/W386.html

Immunogen

Carboxyl fragment of APP 643-695 / Jonas.
Epitope: APP 643-695 C-terminal fragment

Application

Immunohistochemistry on paraffin sections: 10-20 μg/mL * See protocol below.

Western blot: 10 μg/mL

Optimal working dilutions must be determined by end user.

APPLICATION NOTES FOR MAB343

IMMUNOHISTOCHEMISTRY

1) Prepare paraformaldehyde-fixed paraffin sections. Wash twice for 5 min in xylene to deparaffinize. Wash sections for 5 min in a descending series of alcohol solutions (100%, 96%, 90%, 80%, 70%, 50%, 30%).

2) Wash sections 3 times in distilled water.

3) Wash in TBS (50 mM Tris-HCl, 150 mM NaCl, pH 7.6). To block endogenous peroxidase wash with methanol containing 0.6% H2O2 (v/v) and 10 % horse serum (v/v) for 5 min at room temperature.

4) Wash sections for 5 min in TBS.

5) Incubate sections with MAB343 (diluted in TBS containing 10% horse serum (v/v)) overnight at +4°C or for 2 hours at 37°C in a humid chamber.

6) Wash sections 3 times in TBS for 5 min..

7) Detect with standard secondary antibody detection system (PAP, ABC, etc.).

8) Wash sections in TBS.

9) Embed sections and examine.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
This Anti-APP Antibody, APP 643-695 C-terminal fragment, clone 2.F2.19B4 is validated for use in IH(P), WB for the detection of Alzheimer Precursor Protein.

Physical form

Liquid.
Unpurified

Storage and Stability

Maintain lyophilized material at +2–8°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
Brain

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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A TAG1-APP signalling pathway through Fe65 negatively modulates neurogenesis.
Quan-Hong Ma, Toshitaka Futagawa, Wu-Lin Yang, Xiao-Dan Jiang, Li Zeng, Yasuo Takeda et al.
Nature Cell Biology null
Eun Mi Hwang et al.
Bioorganic & medicinal chemistry, 16(14), 6669-6674 (2008-06-21)
In order to access beta-secretase (BACE1), and enzyme strongly implicated in the cause of Alzheimer's disease, inhibitors must possess sufficient lipophilicity to traverse two lipid bilayers. Current drug candidates, which are almost totally peptide-derived, are thus inefficient because cell permeability
Amyloid beta protein precursor and the pathogenesis of Alzheimer's disease.
Selkoe, D J
Cell, 58, 611-612 (1989)
Teresita L Briones et al.
Journal of neurotrauma, 26(4), 613-625 (2009-03-11)
In this study, we examined whether ischemia-induced amyloidogenesis could be modulated by environmental "experience," and whether this modulation is associated with improved cognitive functioning. Rats were subjected to either global ischemia or sham surgery and then were randomly assigned to
Arunabha Chakrabarti et al.
Genomics, proteomics & bioinformatics, 10(4), 208-216 (2012-10-23)
Amyloid precursor protein intracellular domain (AICD) is one of the potential candidates in deciphering the complexity of Alzheimer's disease. It plays important roles in determining cell fate and neurodegeneration through its interactions with several adaptors. The presence or absence of

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