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MAB348

Sigma-Aldrich

Anti-APP A4 Antibody, a.a. 66-81 of APP {NT}, clone 22C11

clone 22C11, Chemicon®, from mouse

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Synonym(s):
APP, Anti - APP A4, Anti-amyloid precursor protein A4
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

22C11, monoclonal

species reactivity

rat, fish, human, mouse, canine, pig, monkey

manufacturer/tradename

Chemicon®

technique(s)

immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... APP(351)

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This Item
MAB343AB1593MABN2287
antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

serum

antibody form

purified immunoglobulin

clone

22C11, monoclonal

clone

2.F2.19B4, monoclonal

clone

polyclonal

clone

1D1, monoclonal

species reactivity

rat, fish, human, mouse, canine, pig, monkey

species reactivity

rat, human

species reactivity

human

species reactivity

human

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

technique(s)

immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

ELISA: suitable, immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

technique(s)

ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

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General description

The β/A4 amyloid precursor protein (APP) is a transmembrane glycoprotein and exists in three isoforms. This protein is composed of a single-pass transmembrane domain, a large extracellular N-terminal domain, and a short cytoplasmic C-terminal tail. The APP gene is located on the human chromosome at 21q21.3-q22.

Specificity

Anti-APP A4 Antibody reacts with pre-A4 and recognizes amino acids 66-81 of the N-terminus on the pre-A4 molecule (Hilbich et al., 1993). 22C11 recognizes all three isoforms of APP, immature ~110kDa, sAPP ~120kDa, and mature ~130kDa (Hoffmann et al., 2000). The Anti-APP A4 antibody is known to cross-react with APLP2 (Slunt, 1994).

Immunogen

Epitope: a.a. 66-81 of APP {N-terminus}
Purified recombinant Alzheimer precursor A4 (pre A4695) fusion protein.

Application

Anti-APP A4 Antibody, a.a. 66-81 of APP , clone 22C11 has been used in immunohistochemistry and immunoblotting(1:1000).

Biochem/physiol Actions

The β/A4 amyloid precursor protein (APP) undergoes proteolytic cleavage and produces β/A4 peptide. The extracellular deposition of this peptide is observed in brain cortical parenchyma and cerebro-meningeal blood vessels which marks the pathological condition of Alzheimer′s disease. Mutations of the APP gene lead to the early onset of Alzheimer′s disease and hereditary cerebral hemorrhage with amyloidosis.

Target description

110/120/130 kDa

Physical form

Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Brain tissue, brain cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(C)

Not applicable


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Protocols

A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.

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