MilliporeSigma
All Photos(2)

Documents

MAB363

Sigma-Aldrich

Anti-NMDAR1 Antibody, clone 54.1

clone 54.1, Chemicon®, from mouse

Sign Into View Organizational & Contract Pricing

Synonym(s):
N-methyl-D-aspartate receptor channel, subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, NMDA receptor 1, glutamate [NMDA] receptor subunit zeta 1, glutamate receptor, ionotropic, N-methyl D-aspartate 1
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

54.1, monoclonal

species reactivity

rat, monkey, human, Xenopus

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
radioimmunoassay: suitable

isotype

IgG2a

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

frog ... Grin1(780048)
human ... GRIN1(2902)
rat ... Grin1(24408)
rhesus monkey ... Grin1(574380)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
AB9864SAB4501301SAB4501302
Anti-NMDAR1 Antibody, clone 54.1 clone 54.1, Chemicon®, from mouse

MAB363

Anti-NMDAR1 Antibody, clone 54.1

species reactivity

rat, monkey, human, Xenopus

species reactivity

rat

species reactivity

mouse, human, rat

species reactivity

rat, mouse, human

clone

54.1, monoclonal

clone

1.17.2.6, monoclonal

clone

polyclonal

clone

polyclonal

biological source

mouse

biological source

rabbit

biological source

rabbit

biological source

rabbit

Gene Information

frog ... Grin1(780048)
human ... GRIN1(2902)
rat ... Grin1(24408)
rhesus monkey ... Grin1(574380)

Gene Information

rat ... Gpr132(314480)

Gene Information

human ... GRIN1(2902)

Gene Information

human ... GRIN1(2902)

isotype

IgG2a

isotype

IgG

isotype

-

isotype

-

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Glutamate is a widely utilized excitatory neurotransmitter in mammalian brain. The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate (KA) and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are referred to as non-NMDA receptors. Two distinct genes encode the NMDA receptor subunits, NR1 and NR2. The NR1 subunit is expressed in abundance in virtually all regions of the brain.

Specificity

Recognizes an epitope between amino acids 660-811 of the NMDAR1 receptor. Shows no cross-reactivity with NMDAR2, NMDAR3, NMDAR4 or NMDAR5.

Immunogen

Epitope: Extracellular loop between transmembrane regions III & IV.
Recombinant fusion protein containing GST and NMDAR1 aa. 660-811.

Application

Anti-NMDAR1 Antibody, clone 54.1 is an antibody against NMDAR1 for use in ELISA, IC, IH, IH(P), RIA.
Immunocytochemistry:
A previous lot of this antibody was used in IC.

RIA:
A previous lot of this antibody was used in RIA.

ELISA:
A previous lot of this antibody was used in ELISA.

Immunohistochemistry: 1:500-2000, on 2-4% paraformaldehyde fixed tissue: typically free-floating, frozen or vibratome sections 24-48 hours at 4°C. Paraffin embedded formalin fixed sections have also been used at similar dilutions and incubation times. Care should be taken not to over fix the tissues. The clone 54.1 has been used for both light and electron microscopy.

Western blot:
1:500 with 50 migrograms/lane of material. Recognizes a 103-116kDa band.

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Quality

Evaluated by immunohistochemistry on rat brain and spinal cord tissue.

Immunohistochemistry(paraffin) Analysis:
NMDAR1 representative staining pattern/morphology on rat spinal cord at 1:200 dilution. Immunoreactivity is seen as integral membrane and nerve fiber staining of neurons. Frozen section. IHC -Frozen Staining Examples: Rat Spinal Cord

Target description

103-116 kDa

Physical form

Format: Purified
Protein A purified
Purified mouse monoclonal IgG2a liquid in buffer PBS, no preservative.

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Rat brain and spinal cord tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Stroke patients develop antibodies that react with components of N-methyl-D-aspartate receptor subunit 1 in proportion to lesion size.
Kalev-Zylinska, ML; Symes, W; Little, KC; Sun, P; Wen, D; Qiao, L; Young, D; During, MJ; Barber, PA
Stroke null
Catherine L Salussolia et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 31(31), 11295-11304 (2011-08-05)
Ionotropic glutamate receptors (iGluRs), including the NMDA receptor subtype, are ligand-gated ion channels critical to fast signaling in the CNS. NMDA receptors are obligate heterotetramers composed of two GluN1 and typically two GluN2 subunits. However, the arrangement of GluN subunits
Csaba Cserép et al.
PloS one, 7(5), e37753-e37753 (2012-06-05)
GABA (gamma-aminobutyric-acid), the main inhibitory neurotransmitter in the adult brain, exerts depolarizing (excitatory) actions during development and this GABAergic depolarization cooperates with NMDARs (N-methyl-D-aspartate receptors) to drive spontaneous synchronous activity (SSA) that is fundamentally important for developing neuronal networks. Although
Transcriptome analysis of synaptoneurosomes identifies neuroplasticity genes overexpressed in incipient Alzheimer's disease.
Williams, C; Mehrian Shai, R; Wu, Y; Hsu, YH; Sitzer, T; Spann, B; McCleary, C; Mo, Y; Miller, CA
Testing null
Taryn N Green et al.
Platelets, 28(8), 799-811 (2017-03-10)
GluN1 is a mandatory component of N-methyl-D-aspartate receptors (NMDARs) best known for their roles in the brain, but with increasing evidence for relevance in peripheral tissues, including platelets. Certain anti-GluN1 antibodies reduce brain infarcts in rodent models of ischaemic stroke.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service