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MAB378

Sigma-Aldrich

Anti-MAP2A Antibody, AP20

ascites fluid, clone AP20, Chemicon®

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Synonym(s):
Microtubule Associated Protein 2
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

AP20, monoclonal

species reactivity

quail, bovine, human, mouse, amphibian, Xenopus, rat, avian

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... MAP2(4133)

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M4403M2320FCMAB318PE
Anti-MAP2A Antibody, AP20 ascites fluid, clone AP20, Chemicon®

MAB378

Anti-MAP2A Antibody, AP20

Gene Information

human ... MAP2(4133)

Gene Information

human ... MAP2(4133)
mouse ... Mtap2(17756)
rat ... Map2(25595)

Gene Information

human ... MAP2(4133)
mouse ... Mtap2(17756)
rat ... Map2(25595)

Gene Information

human ... MAP2(4133)

species reactivity

quail, bovine, human, mouse, amphibian, Xenopus, rat, avian

species reactivity

rat, chicken, human, mouse, bovine, quail

species reactivity

Xenopus, mouse, quail, human, bovine, rat, aquatic salamander

species reactivity

human

clone

AP20, monoclonal

clone

HM-2, monoclonal

clone

AP-20, monoclonal

clone

AP20, monoclonal

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

100

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General description

MAP-2 (microtubule associated protein-2) is on of several high molecular weight proteins that play an important role in brain microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles (Binder, 1984).

MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2 (Caceres, 1984; Binder, 1986). MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds (Caceres, 1986; Dotti, 1987).

Specificity

MAP2a & b. Does not recognize other variants.

Immunogen

Bovine MAP2

Application

Detect MAP2A using this Anti-MAP2A Antibody, AP20 validated for use in IH & WB.
Immunohistochemistry:
1:50-1:200. AP20 stains dendrites and neuron cells bodies but not neuronal processes and axons. A previous lot of this antibody was used in immunohistochemistry.

Western blot:
SDS-PAGE Profiles: In SDS-PAGE MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 300 kD. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-AP20). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier).

Western blot: A 1:500 dilution was used in a previous lot.

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Neurofilament & Neuron Metabolism

Quality

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected MAP 2 on 10 μg of PC12 lysates.

Target description

~ 300 kDa

Linkage

Replaces: MAB3418

Physical form

Unpurified
Unpurified mouse monoclonal IgG1 ascites fluid in buffer containing 15 mM sodium azide

Storage and Stability

Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Control
Brain tissue

Cultured neurons

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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SDF1 reduces interneuron leading process branching through dual regulation of actin and microtubules.
Lysko, DE; Putt, M; Golden, JA
The Journal of Neuroscience null
Morphine and gp120 toxic interactions in striatal neurons are dependent on HIV-1 strain.
Podhaizer, EM; Zou, S; Fitting, S; Samano, KL; El-Hage, N; Knapp, PE; Hauser, KF
Journal of Neuroimmune Pharmacology null
Changqing Xu et al.
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, 11(2), 316-331 (2016-03-20)
In the era of combined antiretroviral therapy (cART), human immunodeficiency virus type 1 (HIV-1) is now considered a chronic disease that specifically targets the brain and causes HIV-1-associated neurocognitive disorders (HAND). Endocannabinoids exhibit neuroprotective and anti-inflammatory properties in several central
Antoinette Defaux et al.
Journal of neuroinflammation, 6, 15-15 (2009-05-09)
Brain inflammation plays a central role in numerous brain pathologies, including multiple sclerosis (MS). Microglial cells and astrocytes are the effector cells of neuroinflammation. They can be activated also by agents such as interferon-gamma (IFN-gamma) and lipopolysaccharide (LPS). Peroxisome proliferator-associated
Seth M Dever et al.
Journal of neurovirology, 18(3), 181-190 (2012-04-25)
The μ-opioid receptor (MOR) is known to undergo extensive alternative splicing as numerous splice variants of MOR have been identified. However, the functional significance of MOR variants, as well as how splice variants other than MOR-1 might differentially regulate human

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