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Key Documents

MAB3864

Sigma-Aldrich

Anti-DNA/Histone H1 Antibody

Chemicon®, from mouse

Synonym(s):

Anti-H1.4, Anti-H1E, Anti-H1F4, Anti-H1s-4, Anti-HIST1H1E, Anti-RMNS, Anti-dJ221C16.5

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable

isotype

IgG2a

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... H1-1(3024)

Specificity

Reacts with DNA/Histone 1. The antibody can be used to stain nuclei of all species and to identify necrosis. By immunohistochemistry, the antibody shows dense staining of nuclei with no staining of nucleoli observed.

Immunogen

Human Raji cell nuclei

Application

Anti-DNA/Histone H1 Antibody is a high quality Mouse Monoclonal Antibody for the detection of DNA/Histone H1 & has been validated in ELISA, FC, ICC & IHC.
Immunocytochemistry: 5-10 μg/mL on 2% paraformaldehyde fixed cells (10 minutes at room temperature)

Immunohistochemistry: 5-10 μg/mL on paraformaldehyde or B5 fixed tissue sections.

Flow cytometry: 5-10 μg/mL using 2% paraformaldehyde fixed cells.

ELISA, direct and indirect

Optimal working dilutions must be determined by end user.

STAINING PATTERN:

Nuclear staining observed. Dense staining of nuclei. No staining of nucleoli.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Physical form

Format: Purified
Protein A purified culture supernatant. Liquid in PBS

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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