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MAB4197

Sigma-Aldrich

Anti-Topoisomerase II Antibody, clone KiS1

clone KiS1, Chemicon®, from mouse

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Synonym(s):
Ki-S1
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

clone

KiS1, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG2a

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TOP2A(7153)

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WH0007153M1ZRB2034MABS2310
antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified antibody

clone

KiS1, monoclonal

clone

1E2, monoclonal

clone

3C3, recombinant monoclonal

clone

TrMab-29, monoclonal

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

mouse

Gene Information

human ... TOP2A(7153)

Gene Information

human ... TOP2A(7153)

Gene Information

human ... TOP2A(7153)

Gene Information

human ... DAG1(1605)

UniProt accession no.

P11388

UniProt accession no.

P11388

UniProt accession no.

P11388

UniProt accession no.

P09758

General description

Topoisomerase II β (M.W 180 kDa) plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is present ubiquitously in normal cells and is upregulated in tumors and proliferating cells. Topoisomerase II β is also implicated in drug resistance of tumor cells.

Specificity

In Immunoprecipitation and Western blot experiments the Ki-S1 antibody recognizes a major protein of 170 kD which was identified as the a isoform of topoisomerase II (Boege et al., 1995; Kreipe et al., 1993). In addition, it has been shown that the Ki-S1 antibody recognizes a carboxyterminal a-isoenzyme specific epitope missing in topoisomerase IIb (Boege et al., 1995). In immunohistochemistry the Ki-S1 antibody shows strong nuclear staining only in proliferating cells. The epitope recognized by the antibody is also detectable in paraffin-embedded tissue sections (Kreipe et al., 1993). Accordingly, it has been shown that the expression of topoisomerase IIa is strongly restricted to proliferating cells (Tsutsui et al., 1993). The topoisomerase IIa antigen is preferentially expressed during G 1 , S, G 2 and M phase of the cell cycle, while resting, non-cycling cells (G 0 phase) lack topoisomerase IIa. In addition, constantly proliferating cells (e.g. cell lines) react positively to topoisomerase IIa during the entire cell-cycle. This specificity of Ki-S1 antibody for proliferating cells might make it a useful tool for determination of the proliferative fraction in solid tumors such as mammary carcinomas (Kreipe et al., 1993; Sampson et al., 1992; Camplejohn et al., 1993; Kreipe et al., 1993; Rasbridge et al., 1994) and gangliomas (Wolf et al., 1994).

Application

Anti-Topoisomerase II Antibody, clone KiS1 is a Mouse Monoclonal Antibody for detection of Topoisomerase II also known as Ki-S1 & has been validated in FC, WB, ICC, IHC, IHC(P).
Western blot: 1-10μg/ml Immunoprecipitaion

Immunocytochemistry: 5-10 μg/ml Immunohistochemistry: 5-10 μg/ml

Flow cytometry

Optimal working dilutions must be determined by end user.

Linkage

Replaces: MABE519

Physical form

Format: Purified

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months.Avoid repeat freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Ki-S1, a novel proliferative marker: flow cytometric assessment of staining in human breast carcinoma cells.
Camplejohn, R S, et al.
British Journal of Cancer, 67, 657-662 (1993)
Kendra Sweet et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 26(1), 54-60 (2019-10-23)
Induction chemotherapy results in complete remission (CR) rates of 20% to 50% among patients with poor-risk AML. Selinexor is an oral selective inhibitor of nuclear export with promising single-agent activity. By inhibiting the primary export protein, XPO1, selinexor localizes and
KiS1--a novel monoclonal antibody which recognizes proliferating cells: evaluation of its relationship to prognosis in mammary carcinoma.
Sampson, S A, et al.
The Journal of Pathology, 168, 179-185 (1992)
H Kreipe et al.
The American journal of pathology, 142(1), 3-9 (1993-01-01)
A monoclonal antibody (Ki-S1) has been raised that reacts with the nuclei of proliferating cells. The antigen recognized is resistant to formalin fixation and can be detected in frozen tissues as well as in routinely processed specimens. In immunohistochemistry, nuclear
N I Valkov et al.
British journal of haematology, 108(2), 331-345 (2000-02-26)
The resistance of several leukaemic and myeloma cell lines (CCRF, L1210, HL-60, KG-1a and RPMI 8226) to VP-16 was found to increase with cell density and to be maximal (3.5- to 39-fold) in plateau phase cell cultures, as measured by

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