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SAB4503053

Sigma-Aldrich

Anti-Ubiquitin antibody produced in rabbit

affinity isolated antibody

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Synonym(s):
RPS27A, UBA52, UBA80, UBCEP1, Ubiquitin
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 8 kDa

species reactivity

mouse, rat, human

concentration

~1 mg/mL

technique(s)

ELISA: 1:20000
immunohistochemistry: 1:50-1:100

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... UBA52(7311)

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U5379SAB1307148SAB1306582
conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

-

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

form

buffered aqueous solution

form

lyophilized powder

form

buffered aqueous solution

form

buffered aqueous solution

technique(s)

ELISA: 1:20000, immunohistochemistry: 1:50-1:100

technique(s)

immunohistochemistry (frozen sections): suitable, western blot: 1:100 using ubiquitin purified from bovine red blood cells

technique(s)

flow cytometry: 1:10-1:50, immunohistochemistry: 1:50-1:100, western blot: 1:1000

technique(s)

immunofluorescence: 1:10-1:50, immunohistochemistry: 1:50-1:100, western blot: 1:1000

UniProt accession no.

P62987

UniProt accession no.

P0CG47

UniProt accession no.

P62987

UniProt accession no.

P0CG48

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General description

Anti-Ubiquitin Antibody detects endogenous levels of total Ubiquitin protein.
Ribosomal protein S27a (RPS27A), also known as ubiquitin carboxyl extension protein 1 (UBCEP-1), is a ribosomal protein, which is naturally synthesized as an ubiquitin (Ub) C-terminal extension protein. The protein is encoded by RPS27A gene mapped to human chromosome 2p16. RPS27 is a 9.8kDa protein characterized with one zinc finger domain in its monomeric state.

Immunogen

The antiserum was produced against synthesized peptide derived from human Ubiquitin.

Immunogen Range: 40-89

Application

Anti-Ubiquitin antibody produced in rabbit has been used in following assays:
  • Western blot analysis.
  • Immunofluorescence-proximity ligation assay (IF-PLA).
  • Immunofluorescence.

Biochem/physiol Actions

Ribosomal protein S27a (RPS27a) is involved in ribosome biogenesis and post-translational modifications of proteins. The encoded protein enhances cell proliferation, controls cell cycle progression, and inhibits apoptosis of leukemia cells. Overexpression of RPS27A or ubiquitin results in advanced-phase chronic myeloid leukemia (CML) and acute leukemia (AL). Hence drugs targeting RPS27a combining with tyrosine kinase inhibitor (TKI) might be a potential treatment for TKI resistant CML patients. Elevated expression of ubiquitin carboxyl extension protein 1 (UBCEP-1), which is a component of ubiquitin pathway, promotes prostate carcinoma development, via increasing the degradation of proteins involved in growth inhibition or apoptosis. RPS27a might act as a unique stress sensor in cells, which results in increased p53 activity leading to inhibition of cell cycle progression.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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Houcai Wang et al.
Oncotarget, 7(14), 18638-18650 (2016-03-05)
STAT3 plays a pivotal role in the hematopoietic system, which constitutively activated by BCR-ABL via JAK and Erk/MAP-kinase pathways. Phospho-STAT3 was overexpressed in imatinib-resistant CML patients as relative to imatinib responsive ones. By activation of the STAT3 pathway, BCR-ABL can
Anti-cancer drug KP1019 induces Hog1 phosphorylation and protein ubiquitylation in Saccharomyces cerevisiae.
Singh V, Azad GK, Reddy M A, et al.
European Journal of Pharmacology, 736, 77-85 (2014)
Houcai Wang et al.
Biochemical and biophysical research communications, 446(4), 1204-1210 (2014-04-01)
Ribosomal protein S27a (RPS27a) could perform extra-ribosomal functions besides imparting a role in ribosome biogenesis and post-translational modifications of proteins. The high expression level of RPS27a was reported in solid tumors, and we found that the expression level of RPS27a
Nagisa Nosrati et al.
Gene, 559(1), 44-51 (2015-01-17)
The small ribosomal protein RPS27a is known to play a role in the activation of cellular checkpoints via p53 which links ribosome biogenesis to cell cycle progression. Here, we show that RPS27a gene is a direct transcriptional target of p53
Santhosh Kumar Sariki et al.
The FEBS journal, 283(22), 4056-4083 (2016-10-26)
Mutations in the Senataxin gene, SETX are known to cause the neurodegenerative disorders, ataxia with oculomotor apraxia type 2 (AOA2), and amyotrophic lateral sclerosis 4 (ALS4). However, the mechanism underlying disease pathogenesis is still unclear. The Senataxin N-terminal protein-interaction and

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