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U5507

Sigma-Aldrich

Ubiquitin human

recombinant, expressed in E. coli (N-terminal histidine tagged)

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CAS Number:
MDL number:
NACRES:
NA.32

biological source

human

Quality Level

recombinant

expressed in E. coli (N-terminal histidine tagged)

assay

≥95% (GE)

form

lyophilized powder

mol wt

10.7 kDa

technique(s)

ligand binding assay: suitable

UniProt accession no.

storage temp.

−20°C

Gene Information

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This Item
U5382T8690ABS2206
Ubiquitin human recombinant, expressed in E. coli (N-terminal histidine tagged)

U5507

Ubiquitin human

Ubiquitin human ≥95% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal FLAG® tagged), lyophilized powder

U5382

Ubiquitin human

Thioredoxin human ≥90% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal histidine tagged), essentially salt-free, lyophilized powder, ≥5 U/mg

T8690

Thioredoxin human

Anti-phospho-Ubiquitin (Ser57)

ABS2206

Anti-phospho-Ubiquitin (Ser57)

biological source

human

biological source

human

biological source

human

biological source

rabbit

assay

≥95% (GE)

assay

≥95% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

assay

-

technique(s)

ligand binding assay: suitable

technique(s)

mass spectrometry (MS): suitable

technique(s)

activity assay: suitable

technique(s)

affinity binding assay: suitable, western blot: suitable

mol wt

10.7 kDa

mol wt

10 kDa

mol wt

-

mol wt

observed mol wt ~28 kDa

form

lyophilized powder

form

lyophilized powder

form

essentially salt-free, lyophilized powder

form

-

General description

Ubiquitin, a globular protein is made of 76 amino acids and has lysine residues on its surface. It has a molecular weight of 8565 Da.

Application

N-Terminal histidine-tagged ubiquitin can replace native ubiquitin in formation of poly-ubiquitin—protein conjugates. The histidine tag enables separation and enrichment of protein conjugates on a Ni(II) column and detection of conjugates in western blot by anti-histidine-tag antibodies.
Ubiquitin human can be used as a test compound for studying the isolation and characterization of rice E3-ubiquitin ligase and the role of OsHOS1 gene in the modulation of the cold stress response.
Ubiquitin human has been used:
  • as a substrate in in vitro ubiquitination assays
  • as substrate in ADP-ribosylation and ubiquitylation assays
  • to supplement the 64 ng of endogenous ubiquitin in fresh blood for experiment grouping in order to study its potential clinical impact on cancer prognosis

Biochem/physiol Actions

Ubiquitin is a small regulatory protein present in eukaryote tissues. Exogenous ubiquitin can stimulate apoptosis in numerous cell lines. E7 protein of human papilloma virus-16 stimulates Retinoblastoma Protein degradation via Ubiquitin-Proteasome Pathway.
Ubiquitin plays a key role in normal eukaryotic cell function. It participates in the development and function of the immune system. This immunophilin is also known to participate in immune homeostasis. The ubiquitin peptide fragment (U50-59) is capable of blocking cellular and humoral immunity.

Preparation Note

Ubiquitin human can dissolved in 0.02 M Tris-HCl at a concentration of 1.00 - 1.10 mg/ml to yield a clear to slightly hazy, colorless solution.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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New crystal form of human ubiquitin in the presence of magnesium
Camara-Artigas A, et al.
Acta Crystallographica. Section F, Structural Biology Communications, 72(1), 29-35 (2016)
Tiago Lourenço et al.
Plant molecular biology, 83(4-5), 351-363 (2013-06-20)
Plants can cope with adverse environmental conditions through the activation of stress response signalling pathways, in which the proteasome seems to play an important role. However, the mechanisms underlying the proteasome-mediated stress response in rice are still not fully understood.
Extracellular Ubiquitin is the Causal Link between Stored Blood Transfusion Therapy and Tumor Progression in a Melanoma Mouse Model
Zhang J, et al.
Journal of Cancer, 10(12), 2822-2835 (2019)
Ubiquitin modification by the E3 ligase/ADP-ribosyltransferase Dtx3L/Parp9
Yang CS, et al.
Molecular Cell, 66(4), 503-516 (2017)
Antigenic mimicry of ubiquitin by the gut bacterium Bacteroides fragilis: a potential link with autoimmune disease
Stewart L, et al.
Clinical and Experimental Immunology, 194(2), 153-165 (2018)

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