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07-2130

Sigma-Aldrich

Anti-Ubiquitin Antibody

from rabbit

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Synonym(s):
Polyubiquitin-C, Ubiquitin
eCl@ss:
32160702

biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, human, mouse

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... UBC(7316)

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Anti-Ubiquitin Antibody from rabbit

07-2130

Anti-Ubiquitin Antibody

Sigma-Aldrich

AB1586

Anti-Ubiquitin Antibody

Anti-Ubiquitin Antibody Upstate®, from rabbit

07-375

Anti-Ubiquitin Antibody

Sigma-Aldrich

AB1690

Anti-Ubiquitin Antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

rat, human, mouse

species reactivity

human, sheep, eukaryotes, rabbit

species reactivity

bovine, human

species reactivity

bovine, eukaryotes, human

Gene Information

human ... UBC(7316)

Gene Information

human ... UBAP2(55833)

Gene Information

human ... UBAP2(55833)

Gene Information

human ... UBAP2(55833)

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

western blot: suitable

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

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General description

Ubiquitin functions as a marker to indicate the fate of other proteins in the cell. It can be attached as a single molecule or as a polyubiquitin chain. Although ubiquitinated proteins are oftentimes tagged for degradation by the 20S proteosome, ubiquitination can also indicate alternative fates depending on the lysine residue that is involved in linking the ubiquitin chain to the target protein.Ubiquitin chains linked by Lys33 to kinase substrates such as AMP-activated protein kinases, (AMPKα1, BRSK1, MARK3, MARK4, and NUAK1), may activate or deactivate these proteins, supporting the role for uniquitin in regulating signaling.

Specificity

Broad species reactivity expected based on sequence similarity.
This antibody recognizes Ubiquitin at the N-terminus

Immunogen

Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the N-terminus of human Ubiquitin.

Application

Research Category
Signaling
Research Sub Category
General Post-translation Modification
This Anti-Ubiquitin antibody is validated for use in IP & WB for the detection of the Ubiquitin protein.
Western Blot Analysis: 0.5 µg/mL from a representative lot detected Ubiquitin on 10 µg of HeLa cell lysate.

Quality

Evaluated by Immunoprecipitation in serum starved HeLa cell lysate.

Immunoprecipitation Analysis: 10 µg of this antibody detected Ubiquitin on 5 µL of serum starved HeLa cell lysate.

Target description

~8 kDa observed. The calculated molecular weight of this protein is 8 kDa, but has been observed at ~24 kDa due to tetramers. (Kvint, K., et al. (2008). Mol Cell.30(4):498-506.)

Physical form

Affinity purified
Format: Purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Serum starved HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Kristian Kvint et al.
Molecular cell, 30(4), 498-506 (2008-05-24)
The final outcome of protein polyubiquitylation is often proteasome-mediated proteolysis, meaning that "proofreading" of ubiquitylation by ubiquitin proteases (UBPs) is crucial. Transcriptional arrest can trigger ubiquitin-mediated proteolysis of RNA polymerase II (RNAPII) so a UBP reversing RNAPII ubiquitylation might be

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