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Anti-Ubiquitinylated proteins Antibody, clone FK1

clone FK1, Upstate®, from mouse

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eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

FK1, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgM

shipped in

wet ice

target post-translational modification

unmodified

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antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

FK1, monoclonal

clone

4.47, monoclonal

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4C8H10, monoclonal

clone

10H7.1, monoclonal

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

isotype

IgM

isotype

IgG1

isotype

IgG1κ

isotype

IgG2aκ

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General description

Ubiquitin is a polypeptide of 76 amino acid residues, being a very highly conserved and widely distributed protein in all eukaryotic cells. It has been proposed that ubiquitin plays important roles in various biological processes such as the regulation of cell cycle, DNA replication, DNA repair, stress response, apoptosis, signal transduction, and the biogenesis of ribosome, nucleosome, peroxisome and myofibrils. In these processes, ubiquitin functions as both a signal for protein degradation and as a chaperone promoting the formation of organelles.

Specificity

Recognizes only poly-ubiquitinylated proteins and not mono-ubiquitinylated proteins or free ubiquitin.

Immunogen

Poly-ubiquitinylated-lysozyme.

Application

Anti-Ubiquitinylated proteins Antibody, clone FK1 is an antibody against Ubiquitinylated proteins for use in WB, IH.
Research Category
Protein Trafficking
Research Sub Category
Ubiquitin & Ubiquitin Metabolism

Physical form

100 µL of concentration 1mg/mL purified immunoglobulin in PBS containing 0.1% sodium azide. Dilute to working strength with phosphate buffered saline pH 7.2-7.4.
Format: Purified

Storage and Stability

Stable for 1 year at 2-8°C from date of shipment for up to 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Chris McKinnon et al.
Acta neuropathologica, 131(3), 411-425 (2015-12-10)
Prion diseases are a group of fatal neurodegenerative disorders characterised by the accumulation of misfolded prion protein (PrP(Sc)) in the brain. The critical relationship between aberrant protein misfolding and neurotoxicity currently remains unclear. The accumulation of aggregation-prone proteins has been
Javier H Jara et al.
Cerebral cortex (New York, N.Y. : 1991), 25(11), 4259-4272 (2015-01-18)
Corticospinal motor neurons (CSMN) receive, integrate, and relay cerebral cortex's input toward spinal targets to initiate and modulate voluntary movement. CSMN degeneration is central for numerous motor neuron disorders and neurodegenerative diseases. Previously, 5 patients with mutations in the ubiquitin
Miao Li et al.
Methods in molecular biology (Clifton, N.J.), 1436, 85-94 (2016-06-02)
Histone deacetylase 6 (HDAC6) is a member of class IIb HDAC family. HDAC6 exists predominantly in the cytoplasm and deacetylates mainly non-histone proteins in the cytoplasm. Via its deacetylase and ubiquitin binding domains, HDAC6 regulates microtubules, cytoskeleton, intracellular trafficking, and
Kunikazu Tanji et al.
Acta neuropathologica communications, 2, 50-50 (2014-06-03)
Extensive research on p62 has established its role in oxidative stress, protein degradation and in several diseases such as Paget's disease of the bone, frontotemporal lobar degeneration and amyotrophic lateral sclerosis. Importantly, previous studies showed that p62 binds directly to
Haopeng Wang et al.
The EMBO journal, 29(7), 1285-1298 (2010-02-13)
Expression of the T-cell receptor (TCR):CD3 complex is tightly regulated during T-cell development. The mechanism and physiological role of this regulation are unclear. Here, we show that the TCR:CD3 complex is constitutively ubiquitylated in immature double positive (DP) thymocytes, but

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