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05-1308

Sigma-Aldrich

Anti-Ubiquitin Antibody, Lys63-Specific, clone Apu3, rabbit monoclonal

clone Apu3, from rabbit

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eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

Apu3, monoclonal

species reactivity

rat, human, mouse, rabbit

technique(s)

cell based assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... UBAP2(55833)

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This Item
05-1313MABS486MABS451
biological source

rabbit

biological source

mouse

biological source

mouse

biological source

mouse

Gene Information

human ... UBAP2(55833)

Gene Information

human ... UBAP2(55833)

Gene Information

human ... UBB(7314)

Gene Information

human ... UBC(7316)

clone

Apu3, monoclonal

clone

HWA4C4, monoclonal

clone

2G7B8, monoclonal

clone

LUB9, monoclonal

species reactivity

rat, human, mouse, rabbit

species reactivity

vertebrates, human

species reactivity

human

species reactivity

human

UniProt accession no.

P0CG47

UniProt accession no.

P62988

UniProt accession no.

P0CG47, P0CG48

UniProt accession no.

P0CG48

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General description

DI-UBIQUITIN PROTEIN AVAILABLE!
Di-Ubiquitin (Lys63-linked) Recombinant Protein (17-10388)
Polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.

In contrast, polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation.

Specificity

Based on sequence homology, broad species cross-reactivity is expected.
Recognizes ubiquitin linked at Lys63. Does not recognize ubiquitin linked at Lys48 or free ubiquitin.

Immunogen

Epitope: Lys63 ubiquitination site
Immunogen used was human di-ubiquitin linked at Lys63.

Application

Immmunoprecipitation:
Rabbit monoclonal anti-ubiquitin (Lys-63-specific), clone Apu3, precipitated ubiquitin-containing proteins from HeLa cell lysates

Immunohistochemistry:
Ubiquitin (Lys63-specific) staining of colorectal cancer tissue sections is restricted to tumor cells.

Flow cytometry:
Flow cytometry analysis of Jurkat cells using Anti-ubiquitin Lys63-specific (#05-1308) (blue) and rabbit IgG control (red).

Inhibition Assay:
Preincubation of anti-ubiquitin, Lys63-specific, with K63-multiubiquitin chains inhibited binding to HeLa cell lysate in Western blots.

Immunocytochemistry:
Ubiquitin (Lys63-specific) staining of colorectal cancer tissue sections is restricted to tumor cells. Positive staining was also detected in mouse, rat, and rabbit brain sections.
Anti-Ubiquitin Antibody, Lys63-Specific, clone Apu3 is a high quality Rabbit Monoclonal Antibody for the detection of Ubiquitin and has been validated in Inhibition Assay (PIA), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC) and Western Blotting (WB).
Research Category
Signaling
Research Sub Category
Ubiquitin & Ubiquitin Metabolism

Quality

Western Blot Analysis:
A 1:1000 dilution of this antibody detected Lys-63 ubiquitin chains by Western blotting, but did not detect free ubiquitin or Lys48 polyubiquitin chains.

Target description

varies

Physical form

Format: Purified
Protein A purified
Protein A purified recombinant rabbit monoclonal antibody that consists of a humanized fAb fragment fused to a rabbit Fc tail produced in a CHO recombinant antibody system. Supplied in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.

Analysis Note

Control
Lys63 polyubiquitin chains.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Atypical ubiquitin chains: new molecular signals. 'Protein Modifications: Beyond the Usual Suspects' review series.
Ikeda, Fumiyo and Dikic, Ivan
EMBO Reports, 9, 536-542 (2008)
Bifunctional apoptosis regulator (BAR), an endoplasmic reticulum (ER)-associated E3 ubiquitin ligase, modulates BI-1 protein stability and function in ER Stress.
Rong, J; Chen, L; Toth, JI; Tcherpakov, M; Petroski, MD; Reed, JC
The Journal of Biological Chemistry null
Unique insights into maternal mitochondrial inheritance in mice.
Luo, SM; Ge, ZJ; Wang, ZW; Jiang, ZZ; Wang, ZB; Ouyang, YC; Hou, Y; Schatten, H; Sun, QY
Proceedings of the National Academy of Sciences of the USA null
Cellular inhibitor of apoptosis (cIAP)-mediated ubiquitination of phosphofurin acidic cluster sorting protein 2 (PACS-2) negatively regulates tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) cytotoxicity.
Guicciardi, ME; Werneburg, NW; Bronk, SF; Franke, A; Yagita, H; Thomas, G; Gores, GJ
Testing null
Gustavo M Silva et al.
Nature structural & molecular biology, 22(2), 116-123 (2015-01-27)
Ubiquitination is a post-translational modification that signals multiple processes, including protein degradation, trafficking and DNA repair. Polyubiquitin accumulates globally during the oxidative stress response, and this has been mainly attributed to increased ubiquitin conjugation and perturbations in protein degradation. Here

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