MilliporeSigma
All Photos(2)

Documents

05-1313

Sigma-Aldrich

Anti-Ubiquitin Antibody, Lys63-Specific, clone HWA4C4

clone HWA4C4, from mouse

Sign Into View Organizational & Contract Pricing

Synonym(s):
ubiquitin C, polyubiquitin B, ubiquitin B
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

HWA4C4, monoclonal

species reactivity

vertebrates, human

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG2a

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... UBAP2(55833)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
17-10388MABS486MABE949
antibody form

purified immunoglobulin

antibody form

-

antibody form

purified antibody

antibody form

purified immunoglobulin

clone

HWA4C4, monoclonal

clone

-

clone

2G7B8, monoclonal

clone

4H8.1, monoclonal

biological source

mouse

biological source

-

biological source

mouse

biological source

mouse

technique(s)

ELISA: suitable, immunohistochemistry: suitable, immunocytochemistry: suitable, western blot: suitable

technique(s)

-

technique(s)

western blot: suitable

technique(s)

western blot: suitable

shipped in

wet ice

shipped in

dry ice

shipped in

wet ice

shipped in

-

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation. In contrast, polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.

Specificity

Based on sequence homology, broad species cross-reactivity is expected.
Recognizes ubiquitin linked at Lys63. Does not recognize ubiquitin linked at Lys48 or free ubiquitin.

Immunogen

Epitope: Lys63 ubiquitination site
Immunogen used was a branched peptide corresponding to the Lys63 ubiquitination site. The C-terminus of Ubiquitin 71-76 peptide (RLRGG) was attached to the K63 in the Ubiquitin 58-63 peptide (CDYNIQKEST).

Application

Anti-Ubiquitin Antibody, Lys63-Specific, clone HWA4C4 is an antibody against Ubiquitin for use in ELISA, IH, IC & WB.
Research Category
Signaling
Research Sub Category
Ubiquitin & Ubiquitin Metabolism
Western Blot Analysis:
A 2 μg/mL dilution of this lot detected Lys-63 ubiquitin chains by Western blotting.
Additional Research Applications

Immunocytochemistry:
This antibody has been reported by an outside laboratory to be suitable for cytochemistry using HEK293T cells transiently transfected with either wild-type ubiquitin or [K63R] ubiquitin.

Immunohistochemistry:
This antibody has been reported by an outside laboratory to be suitable for histochemistry using human brain tissue (hippocampus) from an Alzheimer′s patient.

ELISA:
This antibody has been reported by an outside laboratory to be suitable for ELISA.

Quality

Western Blot Analysis:
A 2 μg/mL dilution of this lot detected Lys-63 ubiquitin chains by Western blotting.

Target description

varies

Physical form

Format: Purified
Purified immunoglobulin by protein G chromatography. Supplied at 1 mg/ml in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.

Analysis Note

Control
Lys63 polyubiquitin chains.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Ubiquitin lys63 is involved in ubiquitination of a yeast plasma membrane protein.
Galan, J M and Haguenauer-Tsapis, R
The Embo Journal, 16, 5847-5854 (1997)
Lys63-linked polyubiquitin chains: linking more than just ubiquitin.
Kawadler, Holli and Yang, Xiaolu
Cancer Biology & Therapy, 5, 1273-1274 (2006)
Xingguang Liu et al.
Cell death and differentiation, 24(4), 683-693 (2017-02-06)
Lung ischemia-reperfusion (I/R) injury remains one of the most common complications after various cardiopulmonary surgeries. The inflammation response triggered by the released damage-associated molecular patterns (DAMPs) aggravates lung tissue damage. However, little is known about the role of autophagy in
Jun Meng et al.
Cell death & disease, 8(6), e2866-e2866 (2017-06-09)
Enterovirus 71 (EV71) is the main causative agent of hand, foot and mouth disease (HFMD), which induces significantly elevated levels of cytokines and chemokines, leading to local or system inflammation and severe complications, whereas the underlying regulatory mechanisms and the
I-Ting Chen et al.
Scientific reports, 5, 12300-12300 (2015-07-21)
Toll-like receptor 4 (TLR4) plays an important role in innate immunity by eliciting inflammation. Upon receptor engagement, transforming growth factor β-activated kinase 1 (TAK1) is an essential mediator that transmits a signal from the receptor to downstream effectors, IκB kinase

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service