MilliporeSigma
All Photos(1)

Documents

U5379

Sigma-Aldrich

Anti-Ubiquitin antibody produced in rabbit

whole antiserum, lyophilized powder

Sign Into View Organizational & Contract Pricing

MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized powder

mol wt

antigen 8.6 kDa

species reactivity

prokaryotes, eukaryotes

technique(s)

immunohistochemistry (frozen sections): suitable
western blot: 1:100 using ubiquitin purified from bovine red blood cells

UniProt accession no.

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
ABS2206SAB4503053MABS486
vibrant-m

ABS2206

Anti-phospho-Ubiquitin (Ser57)

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

mouse

antibody form

whole antiserum

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

purified antibody

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

technique(s)

immunohistochemistry (frozen sections): suitable, western blot: 1:100 using ubiquitin purified from bovine red blood cells

technique(s)

affinity binding assay: suitable, western blot: suitable

technique(s)

ELISA: 1:20000, immunohistochemistry: 1:50-1:100

technique(s)

western blot: suitable

form

lyophilized powder

form

-

form

buffered aqueous solution

form

-

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Ubiquitin (UBB) gene is mapped to human chromosome 17p11.2.
Ubiquitin is a highly conserved, 8.6kDa, eukaryotic cellular protein containing 76 amino acid residues. Ubiquitin is absent in prokaryotes.

Specificity

By immunoblotting, the antibody recognizes 1-3 protein bands using bovine red blood cell ubiquitin.

Immunogen

bovine red blood cell ubiquitin.

Application

Anti-Ubiquitin antibody produced in rabbit is used in:
  • immunostaining in neuropathology
  • immunohistochemistry
  • western blotting

Biochem/physiol Actions

Ubiquitin is involved in the selective degradation of many short-lived proteins by 26S proteasome. Proteins are targeted for degradation by covalent ligation to ubiquitin. This ubiquitin-mediated degradation of regulatory proteins plays crucial roles in the regulation of various processes, such as cell-cycle progression, signal transduction, transcriptional regulation, receptor down-regulation, stress response, protein trafficking and endocytosis. Ubiquitin protein also aids in immune response, metabolism, development, normal protein turnover, protein quality control by degrading misfolded and damaged proteins and apoptosis. Variation in the ubiquitin-mediated processes results in carcinogenesis.

Target description

Ubiquitin, present in both prokaryotes and eukaryotes, is a highly conserved 8.6 kDa protein. It is involved in the selective degradation of many short-lived proteins by the proteasome, an ATP-dependent protease. Proteins are targeted for degradation by covalent ligation to ubiquitin. This ubiquitin-mediated degradation of regulatory proteins plays important roles in the control of numerous processes, including cell-cycle progression, signal transduction, transcriptional regulation, receptor down-regulation, and endocytosis. It has also been implicated in immune response, metabolism, development, protein quality control, and apoptosis.

Physical form

Lyophilized from 0.01 M phosphate buffer, containing 1% lactose.

Reconstitution

Reconstitute the contents of the vial with 10 mL of Tris buffered saline. Since the antisera is preservative free, it is recommended that it be reconstituted in the presence of a preservative or aliquoted and frozen. Avoid repeated freeze/thaw cycles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Mónika Lippai et al.
BioMed research international, 2014, 832704-832704 (2014-07-12)
The ubiquitin-proteasome system and autophagy were long viewed as independent, parallel degradation systems with no point of intersection. By now we know that these degradation pathways share certain substrates and regulatory molecules and show coordinated and compensatory function. Two ubiquitin-like
Andrew Kertesz et al.
Brain : a journal of neurology, 128(Pt 9), 1996-2005 (2005-07-22)
This is a clinicopathologic study of a prospective, clinic-based cohort of patients with frontotemporal dementia (FTD)/Pick complex, who were followed to autopsy. A total of 60 patients with the clinical syndromes of the behavioural variant of FTD (FTD-bv) (n =
Jessica Diring et al.
PloS one, 6(8), e23351-e23351 (2011-08-23)
Alternative splicing and post-translational modifications are processes that give rise to the complexity of the proteome. The nuclear ATF7 and ATF2 (activating transcription factor) are structurally homologous leucine zipper transcription factors encoded by distinct genes. Stress and growth factors activate
Satoshi Sekiguchi et al.
The American journal of pathology, 169(5), 1722-1729 (2006-10-31)
Protein degradation is essential for oogenesis and embryogenesis. The ubiquitin-proteasome system regulates many cellular processes via the rapid degradation of specific proteins. Ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) is exclusively expressed in neurons, testis, ovary, and placenta, each of which has
Rebecca L Welchman et al.
Nature reviews. Molecular cell biology, 6(8), 599-609 (2005-08-03)
Protein ubiquitylation is a recognized signal for protein degradation. However, it is increasingly realized that ubiquitin conjugation to proteins can be used for many other purposes. Furthermore, there are many ubiquitin-like proteins that control the activities of proteins. The central

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service