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MAB5260

Sigma-Aldrich

Anti-Nerve Growth Factor Antibody, clone 27/21

clone 27/21, Chemicon®, from mouse

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Synonym(s):
NGF
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

27/21, monoclonal

species reactivity

human, rat, mouse, bovine

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NTF3(4908)

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MAB5260ZAB5583AB9040
Gene Information

human ... NTF3(4908)

Gene Information

human ... NTF3(4908)

Gene Information

human ... NTF3(4908)

Gene Information

human ... NTF3(4908)

clone

27/21, monoclonal

clone

27/21, monoclonal

clone

polyclonal

clone

polyclonal

species reactivity

human, rat, mouse, bovine

species reactivity

human, rat, bovine, mouse

species reactivity

rat

species reactivity

rat, human, mouse

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

rabbit

UniProt accession no.

P20783

UniProt accession no.

P20783

UniProt accession no.

P20783

UniProt accession no.

P20783

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Specificity

NGF is a neurotrophic protein which is synthesized by the target tissues of NGF-sensitive neurons. NGF acts on sympathetic and sensoric neurons as well as on cholinergic neurons of the basal forebrain. It has been suggested that NGF is involved in the neuropathology of Alzheimer′s Disease (Hefti & Weiner, 1986). In the peripheral nervous system, NGF plays a role in the development of autonomic and sensory neuropathies (Anand et al., 1991).

The antibody specifically reacts with the beta -subunit of NGF from mouse, in the 2.5S- as well as in the 7S-form.

The cross-reactivity with human NGF is 60-90 % of the reactivity with mouse NGF

Immunogen

2.5 S form of mouse NGF.

Application

ELISA

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Neurochemistry & Neurotrophins
This Anti-Nerve Growth Factor Antibody, clone 27/21 is validated for use in ELISA for the detection of Nerve Growth Factor.

Physical form

Format: Purified
Purified immunoglobulin. Lyophilized from 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide. Reconstitute with 1 mL of sterile distilled water.

Storage and Stability

The lyophilized antibody is stable if stored dry at 2-8°C for up to 12 months. After reconstitution maintain at 4°C unaliquotted but sealed tightly for 6 months; do not freeze thaw.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Skull and crossbonesEnvironment

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3


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A Micera et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 35(5), 650-656 (2005-05-19)
Nerve growth factor (NGF) and nerve growth factor receptor (NGFR) expressions have been found to be increased in sub-conjunctival scarring. The aim of this study was to investigate the in vitro effects of NGF on some pro-fibrogenic properties of human
Scott E Counts et al.
Journal of neurochemistry, 113(3), 649-660 (2010-02-06)
Degeneration of locus coeruleus (LC) noradrenergic forebrain projection neurons is an early feature of Alzheimer's disease. The physiological consequences of this phenomenon are unclear, but observations correlating LC neuron loss with increased Alzheimer's disease pathology in LC projection sites suggest
Virginia Protto et al.
Hippocampus, 29(10), 891-904 (2019-03-15)
Diabetes induces early sufferance in the cholinergic septo-hippocampal system, characterized by deficits in learning and memory, reduced hippocampal plasticity and abnormal pro-nerve growth factor (proNGF) release from hippocampal cells, all linked to dysfunctions in the muscarinic cholinergic modulation of hippocampal

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