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MAB5456-C

Sigma-Aldrich

Anti-Gonadotropin-Releasing Hormone Antibody, NT, clone HU11B (Ascites Free)

clone HU11B, from mouse

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Synonym(s):
Gonadotropin-releasing hormone I, GnRH-I, Luliberin I, Luteinizing hormone-releasing hormone I, LH-RH I
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

HU11B, monoclonal

species reactivity

human, rat, rhesus macaque, mouse

technique(s)

affinity binding assay: suitable
electron microscopy: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GNRH1(2796)

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SAB4501228HPA027532MABC567
antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

clone

HU11B, monoclonal

clone

polyclonal

clone

polyclonal

clone

1B4.2, monoclonal

species reactivity

human, rat, rhesus macaque, mouse

species reactivity

mouse, human

species reactivity

rat, human

species reactivity

human

technique(s)

affinity binding assay: suitable, electron microscopy: suitable, immunofluorescence: suitable, immunohistochemistry: suitable (paraffin)

technique(s)

ELISA: 1:1000, immunofluorescence: 1:100-1:500

technique(s)

immunohistochemistry: 1:200-1:500

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

isotype

IgG1κ

isotype

-

isotype

-

isotype

IgG1κ

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General description

Gonadotropin-releasing hormone I (also known as GnRH, GnRH-I, Gonadoliberin-1, Gonadoliberin I, LHRH, LH-RH I, Luteinizing hormone-releasing hormone I) is encoded by the Gnrh1 (also known as Gnrh, Lhrh1, Lnrh) gene (Gene ID 14714) in murine species. GnRH/LHRH is initially produced as a 90-amino acid progonadoliberin-1 precursor protein (UniProt P13562), removal of the signal peptide (a.a. 1-21) yields progonadoliberin-1 (a.a. 22-90), which is further processed by a proteolytic cleavage to release the GnRH/LHRH decapeptide (a.a. 22-31) and the 56-a.a. prolactin release-inhibiting factor 1 (a.a. 35-90). The LHRH decapeptide functions as the primary endocrine link between the brain and the reproductive system. It plays a primary neuroendocrine role in orchestrating the developmental processes associated with sexual development in immature mammals and also in maintaining normal reproductive function in adults. LHRH-producing neurons are located mainly in the lateral anterior hypothalamus, septum, and medial preoptic area, from where they extend axons towards the median eminence and relese the hormone into the hypothalamo-hypophyseal portal blood vessels in an episodic or pulsatile manner.

Specificity

Clone HU11B reacts with C-terminally blocked GnRH-I/LHRH (LHRH-NH2), but not LHRH with a free C-terminal end (LHRH-COOH). Clone HU11B exhibits reduced affinity toward N-terminally truncated LHRH-NH2 and failed to recognize pro-LHRH, Atrial Naturetic Factor/ANF, Growth hormone-releasing hormone/GHRH, Oxytocin, Somatostatin, Thyrotropin-releasing hormone/TRH, and Vasoactive intestinal peptide/VIP (Urbanski, H.F., et al. (1991). Biol. Reprod. 44(4):681-686).

Immunogen

Epitope: N-terminus.
Mouse GnRH (QHWSYGLRPG) conjugated to bovine thyroglobulin.

Application

Anti-Gonadotropin-Releasing Hormone Antibody, NT, clone HU11B (Ascites Free) is an antibody against Gonadotropin-Releasing Hormone for use in Immunohistochemistry (Paraffin), Affinity Binding Assay, Immunofluorescence, Electron Microscopy.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Gonadotropin-Releasing Hormone in human prostate cancer tissue.
Affinity Binding Assay: A representative lot captured C-terminally blocked GnRH-I/LHRH (LHRH-NH2), but not LHRH with a free C-terminal end (LHRH-COOH). Clone HU11B exhibited reduced affinity toward N-terminally truncated LHRH-NH2 and failed to recognize pro-LHRH, Atrial Naturetic Factor/ANF, Growth hormone-releasing hormone/GHRH, Oxytocin, Somatostatin, Thyrotropin-releasing hormone/TRH, and Vasoactive intestinal peptide/VIP (Urbanski, H.F., et al. (1991). Biol. Reprod. 44(4):681-686).
Immunofluorescence Analysis: A representative lot detected GnRH neurons in brain sections from female rhesus monkeys by fluorescent immunohistochemistry (Naugle, M.M., and Gore, A.C. (2014). Neuroendocrinology 100:334-346).
Immunofluorescence Analysis: A representative lot detected pregnancy stage-dependent GnRH-I immunoreactivity in the luminal epithelium of rat oviduct by fluorescent immunohistochemistry using paraformaldehyde-fixed cryosections (Sengupta, A., et al. (2007). J. Histochem. Cytochem. 55(5):525-534).
Immunofluorescence Analysis: A representative lot detected GnRH-I immunoreactivity in frozen mouse hypothalamic sections by fluorescent immunohistochemistry (Olcese, J., et al. (2003). Neuroreport. 14(4):613-618).
Electron Microscopy: A representative lot detected GnRH-I immunoreactivity associated with the luminal epithelium secretory vesicles of rat oviduct by EM using paraformaldehyde-fixed LR wihite sections from rats at day 16 of pregnancy (Sengupta, A., et al. (2007). J. Histochem. Cytochem. 55(5):525-534).
Immunohistochemistry Analysis: A representative lot detected GnRH-I immunoreactivity throughout the hypothalamus in paraformalin-fixed, paraffin-embedded South American plains vizcacha (Lagostomus maximus) brain sections (Dorfman, V.B., et al. (2011). J. Mol. Histol. 42(4):311-321).
Immunohistochemistry Analysis: A representative lot detected GnRH-I-positie neurons in formalin-fixed, paraffin-embedded mouse brain sections (Bergman, J.E., et al. (2010). Eur. J. Hum. Genet. 18(2):171-177).
Immunohistochemistry Analysis: A representative lot detected GnRH-I immunoreactivity in paraformaldehyde-fixed rat brain vibratome sections (Urbanski, H.F., et al. (1991). Biol. Reprod. 44(4):681-686).
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Immunohistochemistry in human prostate tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Gonadotropin-Releasing Hormone in human prostate tissue.

Target description

10 kDa calculated

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Zixuan Gao et al.
Journal of ovarian research, 13(1), 106-106 (2020-09-15)
The exact pathogenesis of polycystic ovary syndrome (PCOS), the most common neuroendocrine disorder in women of reproductive age, has not been fully elucidated. Recent studies suggested that chronic inflammation and neurotransmitter disorder involved in the progress of PCOS. Troxerutin, a

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