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MAB8052

Sigma-Aldrich

Anti-Adenovirus Antibody, clone 20/11

clone 20/11, Chemicon®, from mouse

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eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

clone

20/11, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

isotype

IgG1

shipped in

wet ice

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This Item
MAB8051MAB805MAB8043
biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

clone

20/11, monoclonal

clone

2/6, monoclonal

clone

2/6, monoclonal, 20/11, monoclonal

clone

7/48.7c, monoclonal

technique(s)

ELISA: suitable, immunofluorescence: suitable, flow cytometry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

technique(s)

ELISA: suitable, flow cytometry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable

technique(s)

ELISA: suitable, immunofluorescence: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

technique(s)

immunofluorescence: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

Adenoviruses are 65-80 nm, non-enveloped, regular icosahedron pathogens often associated with respiratory and gastrointestinal illness as well as conjunctivitis. Well over 40 types of adenoviruses have currently been recognized. In addition to their deleterious effects, adenoviruses have been used in vaccine production and in gene therapy. They also have the ability to trigger cell proliferation by interfering with host cellular anti-oncogenes. Since they are easy to prepare, multiple quickly and efficiently, and have the ability to infect and deliver their genome (including researcher-created constructs) to the nucleus of a variety of post-mitotic cells, adenoviruses are extremely valuable in research and medical applications ranging from virus/host cell interaction to the insertion of novel genes or regulatory mechanisms into eukaryotic cellular systems, to delivery of therapeutic anti-cancer genes.

Specificity

Recognizes All 41 serotypes of Adenovirus.

Immunogen

Adeno 3.

Application

Anti-Adenovirus Antibody, clone 20/11 detects level of Adenovirus & has been published & validated for use in ELISA, FC, IF, IH, IH(P).
EIA, IFA

Immunohistochemistry: 1:200. In trypsinized (with 0.1% trypsin in Tris buffer pH 7.6 containing 0.1% Calcium Cloride; for 5-10 minutes at room temp.) paraffin embedded tissue. Do not use antigen retrieval.

Flow cytometry.

Final working dilutions and protocols must be determined by end user.
Research Category
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral

Physical form

Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified

Storage and Stability

Maintain at +2–8°C in convenient aliquots for up to 12 months. Do not store in a diluted format.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Analysis Note

Control
Adenovirus Control Slides, Catalogue Number 5009-5

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Gabriel Girouard et al.
Emerging infectious diseases, 19(1), 119-122 (2012-12-25)
We describe 3 culture-proven cases of adenovirus serotype 14 infection in New Brunswick, Canada, during the summer of 2011. Strains isolated from severely ill patients were closely related to strains of a genomic variant, adenovirus 14p1, circulating in the United
Amitinder Kaur et al.
Virology, 357(2), 199-214 (2006-09-12)
The immunogenicity and protective capacity of replication-defective herpes simplex virus (HSV) vector-based vaccines were examined in rhesus macaques. Three macaques were inoculated with recombinant HSV vectors expressing Gag, Env, and a Tat-Rev-Nef fusion protein of simian immunodeficiency virus (SIV). Three
Simultaneous occurrence of lymphoepithelial cysts, cytomegalovirus and mycobacterial infections in the intraparotid lymph nodes of a patient with AIDS.
P A Vargas, H Villalba, A P Passos, P H Saldiva, T Mauad, H H Caiaffa Filho, A Lucena, O P Almeida
Journal of Oral Pathology & Medicine null
Shady Younis et al.
Proceedings of the National Academy of Sciences of the United States of America, 115(16), E3808-E3816 (2018-04-04)
The zinc finger CCCH-type containing 11A (ZC3H11A) gene encodes a well-conserved zinc finger protein that may function in mRNA export as it has been shown to associate with the transcription export (TREX) complex in proteomic screens. Here, we report that
Late-onset acute haemorrhagic necrotizing granulomatous adenovirus tubulointerstitial nephritis in a renal allograft.
Alsaad, KO; Tobar, A; Belanger, E; Ahmad, M; Cattran, DC; Herzenberg, AM
Nephrology, Dialysis, and Transplantation null

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