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MAB8052

Sigma-Aldrich

Anti-Adenovirus Antibody, clone 20/11

clone 20/11, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

clone

20/11, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

isotype

IgG1

shipped in

wet ice

General description

Adenoviruses are 65-80 nm, non-enveloped, regular icosahedron pathogens often associated with respiratory and gastrointestinal illness as well as conjunctivitis. Well over 40 types of adenoviruses have currently been recognized. In addition to their deleterious effects, adenoviruses have been used in vaccine production and in gene therapy. They also have the ability to trigger cell proliferation by interfering with host cellular anti-oncogenes. Since they are easy to prepare, multiple quickly and efficiently, and have the ability to infect and deliver their genome (including researcher-created constructs) to the nucleus of a variety of post-mitotic cells, adenoviruses are extremely valuable in research and medical applications ranging from virus/host cell interaction to the insertion of novel genes or regulatory mechanisms into eukaryotic cellular systems, to delivery of therapeutic anti-cancer genes.

Specificity

Recognizes All 41 serotypes of Adenovirus.

Immunogen

Adeno 3.

Application

Anti-Adenovirus Antibody, clone 20/11 detects level of Adenovirus & has been published & validated for use in ELISA, FC, IF, IH, IH(P).
EIA, IFA

Immunohistochemistry: 1:200. In trypsinized (with 0.1% trypsin in Tris buffer pH 7.6 containing 0.1% Calcium Cloride; for 5-10 minutes at room temp.) paraffin embedded tissue. Do not use antigen retrieval.

Flow cytometry.

Final working dilutions and protocols must be determined by end user.
Research Category
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral

Physical form

Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified

Storage and Stability

Maintain at +2–8°C in convenient aliquots for up to 12 months. Do not store in a diluted format.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Analysis Note

Control
Adenovirus Control Slides, Catalogue Number 5009-5

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Late-onset acute haemorrhagic necrotizing granulomatous adenovirus tubulointerstitial nephritis in a renal allograft.
Alsaad, KO; Tobar, A; Belanger, E; Ahmad, M; Cattran, DC; Herzenberg, AM
Nephrology, Dialysis, and Transplantation null
Caihong Chen et al.
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, 32(6), 893-898 (2012-12-29)
Conditionally replication adenovirus M4, which was constructed in our lab, was proved to have good clinical application prospect for its good anti-tumor and anti-metastasis effect. However, clinically applying M4 faces many problems. One of the most important is the safety
Coagulation factor IX mediates serotype-specific binding of species A adenoviruses to host cells.
Lenman, A; Muller, S; Nygren, MI; Frangsmyr, L; Stehle, T; Arnberg, N
Journal of virology null
Wael Kamel et al.
RNA (New York, N.Y.), 23(11), 1700-1711 (2017-08-26)
Here we show that the adenovirus major late promoter produces a 31-nucleotide transcriptional start site small RNA (MLP-TSS-sRNA) that retains the 7-methylguanosine (m7G)-cap and is incorporated onto Ago2-containing RNA-induced silencing complexes (RISC) in human adenovirus-37 infected cells. RNA polymerase II
Detection and quantitation of infectious human adenoviruses and JC polyomaviruses in water by immunofluorescence assay.
Calgua B, Barardi CR, Bofill-Mas S, Rodriguez-Manzano J, Girones R
Journal of Virological Methods null

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