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ZMS1040

Sigma-Aldrich

Anti-Adenovirus Hexon Antibody, clone 7/48.7c ZooMAb® Mouse Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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biological source

mouse

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

7/48.7c, recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 109.15 kDa

purified by

using protein G

species reactivity

virus

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgG1

epitope sequence

Unknown

Protein ID accession no.

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

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This Item
ZMS1110ZRB1384ZMS1063
clone

7/48.7c, recombinant monoclonal

clone

20/11, recombinant monoclonal

clone

1N5, recombinant monoclonal

clone

AE1, recombinant monoclonal

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

species reactivity

virus

species reactivity

adenovirus-infected cells

species reactivity

human

species reactivity

human

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

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General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 7/48.7c is a ZooMAb® Mouse recombinant monoclonal antibody that detects Adenovirus hexon protein.

Immunogen

Purified Adenovirus 1,2,5,6 Hexon protein.

Application

Quality Control Testing

Evaluated by Immunocytochemistry in A549 cells infected with Adenovirus Infection.

Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected Adenovirus Hexon in A549 cells infected with Adenovirus Infection.

Tested applications

Flow Cytometry Analysis: 0.1 μg from a representative lot detected Adenovirus Hexon in one million A549 cells infected with Adenovirus.

Western Blotting Analysis: A representative lot detected Adenovirus Hexon in Western Blotting applications (Wu, Hongju, et. al. (2002). J Virol. 76(24):12775-82).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Adenovirus Hexon, clone 7/48.7c ZooMAb®, Cat. No. ZMS1040, is a recombinant Mouse monoclonal antibody that detects Adenovirus Hexon protein and is tested for use in Flow Cytometry, Immunocytochemistry, and Western Blotting.

Target description

Hexon protein (UniProt: P03277; also known as CP-H, Protein II) is encoded by L3 gene (Gene ID: 2652998) in Adenovirus. Adenoviruses are large (65-80 nm), non-enveloped viruses containing a dsDNA genome. They typically cause mild infections involving the upper or lower respiratory tract, gastrointestinal (GI) tract, or conjunctiva. However, rare manifestations of adenovirus infections may include hemorrhagic cystitis, hepatitis, hemorrhagic colitis, pancreatitis, nephritis, or encephalitis. They also are used gene delivery vectors. They also have the ability to trigger cell proliferation by interfering with host cellular anti-oncogenes. The principal component of adenovirus is the homotrimeric hexon and there are 240 on the faces and edges of the capsid with the pentons consisting of the penton bases and extended fibers on the 12 fivefold apices. The size of the hexon molecule can vary with the serotype. The base of each hexon molecule has one loop and two eight-stranded jelly rolls that are important for interacting with neighboring capsomeres. Other so-called minor components: IIIa, VI, VIII and IX are also associated with the capsid. There are six other structural components situated in the virus core, of which five are associated with the double stranded DNA genome and the remaining component is the 23K virion protease that plays a vital role in the assembly of the virion. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant mouse monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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