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MABC83

Sigma-Aldrich

Anti-Vps4/SKD1 Antibody, clone 5E6.1

clone 5E6.1, from mouse

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Synonym(s):
Vacuolar protein sorting-associated protein 4B, Cell migration-inducing gene 1 protein, Suppressor of K(+) transport growth defect 1, Protein SKD1
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5E6.1, monoclonal

species reactivity

mouse, human

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... VPS4B(9525)

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Anti-VPS4A Antibody, clone 14B1.1 clone 14B1.1, from mouse

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Anti-VPS4A Antibody, clone 14B1.1

clone

5E6.1, monoclonal

clone

polyclonal

clone

polyclonal

clone

14B1.1, monoclonal

Gene Information

human ... VPS4B(9525)

Gene Information

human ... VPS4B(9525)

Gene Information

human ... VPS4B(9525)

Gene Information

human ... VPS4A(27183)

biological source

mouse

biological source

rabbit

biological source

rabbit

biological source

mouse

antibody form

purified immunoglobulin

antibody form

serum

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

immunoprecipitation (IP): 10-20 μg using lysate of mouse 3T3 cells, western blot: 2-4 μg/mL using whole extracts of G361 cells

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

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General description

Vacuolar protein sorting-associated protein 4B (Vps4), or SKD1, is a ubiquitous AAA ATPase involved in endosomal vesicle trafficking. Trafficking of vesicles containing proteins such as receptors, from the endosome to the cytoplasm, is an important regulatory mechanism for cell-surface receptors and the signaling pathways that involve those receptors. Such endosomal vesicles are shuttled from the endosome to the cytoplasm by multivesicular bodies (MVB). MVBs are large multi-protein complexes that assemble and disassemble during this process. Previous studies have indicated that the yeast homologue of Vps4, Vps4p (60 % sequence identity to Vps4), interacts with endosomes via its N-terminal microtubule interacting and trafficking (MIT) domain, and also associates with a number of MVB proteins including Vps2p, Bro1p, Vps20p, Vta1p, and Did2p/Chm1p. Although the precise role of Vps4 is still unclear, previous studies have suggested that it interacts with the ESCRT-III complex involved in the sorting of MVBs and has been associated with virus-loaded vesicles such those in HIV-infected cells.

Immunogen

Linear peptide corresponding to human Vps4/SKD1.

Application

Anti-Vps4/SKD1 Antibody, clone 5E6.1 is an antibody against Vsp4/SKD1 for use in Western Blotting, ICC.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Vps4/SKD1 in NIH/3T3, A431, and HeLa cells.
Research Category
Apoptosis & Cancer
Research Sub Category
Cell Cycle, DNA Replication & Repair

Quality

Evaluated by Western Blot in human skeletal muscle tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Vps4/SKD1 in 10 µg of human skeletal muscle tissue lysate.

Target description

~52 kDa observed. The calculated molecular weight of this protein is ~50 kDa Uniprot describes that this protein is autophosphoralated upon DNA damage.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human skeletal muscle tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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