MABC86
Anti-Nesprin-2 Antibody, clone K20-478
clone K20-478, from mouse
Synonym(s):
Nesprin-2, Nuclear envelope spectrin repeat protein 2, Nucleus and actin connecting element protein, Protein NUANCE, Synaptic nuclear envelope protein 2, Syne-2
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About This Item
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
K20-478, monoclonal
species reactivity
human, mouse
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
General description
Immunogen
Application
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Nesprin-2 in HA-MKS3 transfected HEK293 whole cell extracts. Immunoprecipitated sample was then subjected to Western Blotting using the same representative lot of antibody (Dawe, H. R., et al. (2009). J Cell Sci. 122(Pt 15): 2716-2626.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Nesperin-2 in IMCDE whole cell extracts. High levels of a 25 kDa ABD-containing isoform was observed only at post-confluence (+72 hours); however, a second major ABD-containing isoform was observed at 55 kDa before and after confluence (Dawe, H. R., et al. (2009). J Cell Sci. 122(Pt 15): 2716-2626.).
Dylight® is a registered trademark of Thermo Fisher Scientific. Alexa Fluor is a registered trademark of Molecular Probes, Inc.
Cell Structure
Cytoskeleton
Quality
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Nesprin-2 in 10 µg of COS-7 cell lysate.
Target description
Physical form
Storage and Stability
Other Notes
Legal Information
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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