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MABD122

Sigma-Aldrich

Anti-CD73 Antibody, clone AA60-E3-3

clone AA60-E3-3, from mouse

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Synonym(s):
5′-nucleotidase, 5′-NT, ecto-5′-nucleotidase
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

AA60-E3-3, monoclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (immunogen homology)

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NT5E(4907)

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This Item
MABD122A4SAB4701116SAB4700863
biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

species reactivity

human

species reactivity

human

species reactivity

-

species reactivity

human

clone

AA60-E3-3, monoclonal

clone

AA60-E3-3, monoclonal

clone

AD2, monoclonal

clone

AD2, monoclonal

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

UniProt accession no.

P21589

UniProt accession no.

Q61503

UniProt accession no.

-

UniProt accession no.

-

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General description

CD73, also known as Ecto-5′-nucleotidase, catalyzes the conversion of purine 5′ mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation, being expressed on subsets of B-cells and T-cells. CD73 is also a marker of undifferentiated mesenchymal stem cells and is often used to identified stem cell populations.

Immunogen

Whole Mouse Dermal Lymphatic Endothelial Cells (DLEC)

Application

Detect CD73 using this mouse monoclonal antibody, Anti-CD73 Antibody, clone AA60-E3-3 validated for use in ICC, Flow Cytometry, western blotting & IP.
Research Category
Stem Cell Research
Research Sub Category
Mesenchymal Stem Cells
This antibody has been shown to work in immunocytochemical applications on human adipose mesenchymal stem cells. This antibody has been shown to work in western blot applications on HMEC-1 cells by an independant lab.This antibody has been shown to work in immunoprecipitation applications on HMEC-1 cells by an independant lab.

Quality

Evaluated by Flow Cytometry on human adipose mesenchymal stem cells.
Flow Cytometry Analysis: 1 µg/mL of this antibody detected CD73 on 1X10^6 human adipose mesenchymal stem cells.

Target description

63 kDa calculated

Linkage

Replaces: MABC668

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human Adipose Mesenchymal Stem Cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Said A Goueli et al.
PloS one, 14(10), e0220094-e0220094 (2019-10-28)
The success of immunotherapy treatment in oncology ushered a new modality for treating a wide variety of cancers. However, lack of effect in some patients made it imperative to identify other pathways that are exploited by cancer cells to circumvent
Anthony W Tolcher et al.
Journal for immunotherapy of cancer, 11(2) (2023-02-07)
Cluster of differentiation (CD)73-adenosine and transforming growth factor (TGF)-β pathways are involved in abrogated antitumor immune responses and can lead to protumor conditions. This Phase 1 study (NCT03954704) evaluated the safety, pharmacokinetics, pharmacodynamics, and efficacy of dalutrafusp alfa (also known
David Tobys et al.
Traffic (Copenhagen, Denmark), 22(1-2), 6-22 (2020-11-24)
In eukaryotic cells, clathrin-mediated endocytosis (CME) is a central pathway for the internalization of proteins from the cell surface, thereby contributing to the maintenance of the plasma membrane protein composition. A key component for the formation of endocytic clathrin-coated vesicles
Mahmoud Yousefifard et al.
Journal of stem cells & regenerative medicine, 18(2), 53-63 (2023-01-31)
Few studies are conducted on the efficacy of human adipose-derived stem cells (ADSCs) in spinal cord injury (SCI) management and electrophysiological changes in the spinal cord. Therefore, the present study aimed to determine the effect of ADSCs on neuropathic pain
Giulia Detela et al.
Biotechnology journal, 13(2) (2018-01-16)
Human mesenchymal stromal cells (hMSCs) are excellent candidates for cell therapy but their expansion to desired clinical quantities can be compromised by ex vivo processing, due to differences between donor material and process variation. The aim of this article is

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PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.

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