MilliporeSigma
All Photos(2)

Documents

MABS132

Sigma-Aldrich

Anti-GLUT1 (CT) Antibody, clone 5B12.3

clone 5B12.3, from mouse

Sign Into View Organizational & Contract Pricing

Synonym(s):
Solute carrier family 2 facilitated glucose transporter member 1, Glucose transporter type 1,erythrocyte/brain, GLUT-1, HepG2 glucose transporter
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5B12.3, monoclonal

species reactivity

human, rat

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... SLC2A1(6513)

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SAB4502803ABN991SAB5500114
clone

5B12.3, monoclonal

clone

polyclonal

clone

polyclonal

clone

SP168, monoclonal

Gene Information

human ... SLC2A1(6513)

Gene Information

human ... SLC2A1(6513)

Gene Information

human ... SLC2A1(6513)

Gene Information

human ... SLC2A1(6513)

species reactivity

human, rat

species reactivity

mouse, human, rat

species reactivity

mouse, human

species reactivity

human (tested)

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

GLUTs (Glucose transporters) are a family of integral membrane proteins that facilitative glucose uptake by cells. They are integral membrane glycoproteins involved in transporting glucose into most cells. Seven types of glucose transport carrier proteins, designated as GLUT 1 to 7, facilitate glucose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encode at least 7 proteins exhibiting high degree of amino acid homology (45% to 65%), all in the molecular weight range of 40 to 60 kDa. GLUT1 functions in constitutive or basal glucose uptake in adult tissues and is the predominant glucose transporter in embryonic and fetal tissues. It has two forms, a 55 kDa and a 45 kDa. The 55 kDa form of GLUT1 regulates import of glucose from blood to brain across the endothelial cells of the blood-brain barrier (BBB), whereas the 45 kDa form of GLUT1 predominantly regulates nonvascular glial glucose uptake.

Specificity

This antibody recognizes GLUT1 at the C-terminus.

Immunogen

Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human GLUT1.

Application

Anti-GLUT1 (C-terminus) Antibody, clone 5B12.3 is an antibody against GLUT1 (C-terminus) for use in Western Blotting & IHC.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected GLUT1 in rat cerebellum and human cerebellum tissues.
Research Category
Signaling
Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blot in NGF treated PC-12 cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected GLUT1 in 10 µg of NGF treated PC-12 cell lysate.

Target description

~54 kDa observed

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
NGF treated PC-12 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Stanley Andrisse et al.
American journal of physiology. Endocrinology and metabolism, 314(4), E353-E365 (2018-01-20)
Hyperandrogenemia and hyperinsulinemia are believed to play prominent roles in polycystic ovarian syndrome (PCOS). We explored the effects of low-dose dihydrotestosterone (DHT), a model of PCOS, on insulin signaling in metabolic and reproductive tissues in a female mouse model. Insulin
Linn Stenfelt et al.
Transfusion, 59(3), 1108-1117 (2019-01-01)
The P1 antigen was first described in 1927 and belongs to the P1PK histo-blood group system, together with Pk and NOR. The A4GALT-encoded 4-α-galactosyltransferase synthesizes these antigens and has been considered to extend glycolipids exclusively. However, contradicting studies have been
Jiaming Zhou et al.
ASN neuro, 15, 17590914231151534-17590914231151534 (2023-02-18)
Retinitis pigmentosa (RP) is an inherited disorder that results in vision impairment but general and mutation-independent therapeutic strategies are not available. However, it is widely regarded that the cGMP system, including cGMP and its interactor cGMP-dependent protein kinase (PKG), acts
PET Demonstrates Functional Recovery after Treatment by Danhong Injection in a Rat Model of Cerebral Ischemic-Reperfusion Injury.
Wang, Z; Song, F; Li, J; Zhang, Y; He, Y; Yang, J; Zhou, H; Zhao, T; Fu, W; Xing, P; Wan et al.
Evidence-Based Complementary and Alternative Medicine : ECAM null
Roberta Battistella et al.
International journal of molecular sciences, 22(21) (2021-11-14)
The vascular system is vital for all tissues and the interest in its visualization spans many fields. A number of different plant-derived lectins are used for detection of vasculature; however, studies performing direct comparison of the labeling efficacy of different

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service