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MABE1132

Sigma-Aldrich

Anti-TET2 Antibody, clone 9F7

clone 9F7, from rat

Synonym(s):

Methylcytosine dioxygenase TET2, Protein Ayu17-449

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9F7, monoclonal

species reactivity

mouse

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_001035490

UniProt accession no.

Q4JK59

target post-translational modification

unmodified

Gene Information

mouse ... Tet2(214133)

General description

Methylcytosine dioxygenase TET2 (EC 1.14.11.n2; UniProt Q4JK59; also known as Protein Ayu17-449) is encoded by the Tet2 (also known as Kiaa1546) gene (Gene ID 214133) in murine species. TET (ten-eleven translocation) proteins (TET1, TET2, and TET3) oxidize 5-methylcytosine to 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine, providing a means for active epigenetic DNA demethylation in mammals. TET proteins also modify genomic thymine residues to 5-hydroxyuracil. TET proteins depend on Fe(II) and 2-oxoglutarate as cofactors for activity, and 2-oxoglutarate is known to inhibit their activity, while ascorbate is shown to stimulate TET-mediated cytosine oxidation. In addition, post-translational modifications (PTMs), such as phosphorylation and O-GlcNAcylation, also play a role in regulating the activity of TET proteins. TET1 and TET2 are found highly expressed in mouse embryonic stem cells (mESCs) and TET3 is known to be upregulated in oocytes and oxidize the silenced paternal pronuclear DNA. High levels of TET proteins and genomic 5-hydroxymethylcytosine in neuronal tissues are also reported.

Specificity

Clone 9F7 detected exogenously expressed TET2, but not TET1 or TET3, GST fusion (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812). Epitope region is present in all three murine TET2 spliced isoforms reported by UniProt (Q4JK59).

Immunogen

Epitope: Region between DNA-binding and 2-oxoglutarate-binding domains.
His-tagged recombinant fragment from the region between DNA-binding and 2-oxoglutarate-binding domains of mouse TET2.

Application

Anti-TET2 Antibody, clone 9F7 is an antibody against TET2 for use in Immunocytochemistry, Western Blotting, Immunoprecipitation, ELISA.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Western Blotting Analysis: A representative lot detected exogenously expressed TET2, but not TET1 or TET3, GST fusion in lysates from respective HEK293T transfectants (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
Immunoprecipitation Analysis: A representative lot immunoprecipitated endogenous TET2 from murine embryonic stem cell (mESC) lysate. Subsequent LC-MS/MS analysis detected the presence of co-immunoprecipitated glycosyltransferase OGT (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
ELISA Analysis: Clone 9F7 hybridoma culture supernatant was confirmed for its immunoreactivity against His-tagged murine TET2 by ELISA (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).

Quality

Evaluated by Immunocytochemistry in mouse mouse ESCs.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected TET2 in mouse embryonic stem cells (ESCs).

Target description

212.1 kDa (isoform 1), 68.06 kDa (isoform 2), 64.50 kDa (isoform 3) calculated.

Physical form

Format: Purified
Protein G purified.
Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Szymon J Szymura et al.
BMC biology, 18(1), 32-32 (2020-03-27)
Nuclear factor-κB (NF-κB) plays a prominent role in promoting inflammation and resistance to DNA damaging therapy. We searched for proteins that modulate the NF-κB response as a prerequisite to identifying novel factors that affect sensitivity to DNA damaging chemotherapy. Using
Guo-Dong Chen et al.
Science advances, 9(2), eabo7605-eabo7605 (2023-01-12)
Execution of lineage-specific differentiation programs requires tight coordination between many regulators including Ten-eleven translocation (TET) family enzymes, catalyzing 5-methylcytosine oxidation in DNA. Here, by using Keratin 14-Cre-driven ablation of Tet genes in skin epithelial cells, we demonstrate that ablation of

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