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MABE285

Sigma-Aldrich

Anti-Replication Protein A Antibody, clone RPA34-20

clone RPA34-20, from mouse

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Synonym(s):
Replication protein A 32 kDa subunit, RP-A p32, Replication factor A protein 2, RF-A protein 2, Replication protein A 34 kDa subunit, RP-A p34
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

RPA34-20, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... RPA2(6118)

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MABE286PLA0310PLA0071
Gene Information

human ... RPA2(6118)

Gene Information

human ... RPA2(6118)

Gene Information

human ... RPA2(6118)

Gene Information

human ... RPA2(6118)

clone

RPA34-20, monoclonal

clone

RPA34-19, monoclonal

clone

-

clone

-

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human, mouse

antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

affinity purified immunoglobulin

antibody form

affinity purified immunoglobulin

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

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General description

RPA (RP-A p32) is a heterotrimeric protein complex that binds specifically to single-stranded DNA (ssDNA). It is composed of three subunits: RPA1 (70 kDa), RPA2 (32 kDa), and RPA3 (14 kDa). RPA plays multiple roles in DNA replication. At the onset of DNA replication, RPA is loaded onto chromatin, and is needed for subsequent loading of DNA polymerase and other replication proteins to initiate DNA replication. After replication begins, RPA moves with replication forks, stabilizing ssDNA and assisting in DNA synthesis. In addition to its replication function, RPA is also known to play essential roles in damage repair and recombination. The 32 kDa subunit is phosphorylated by the cdc2 family of kinases when cells enter S-phase; and by ATM, ATR, and DNA-PK proteins in response to DNA damage.

Immunogen

Replication Protein A purified from U293 cells

Application

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Replication Protein A in HeLa and A431 cells.

Immunohistochemistry Analysis: A 1:5 dilution from a representative lot detected Replication Protein A in human colorectal adenocarcinoma tissues.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Use Anti-Replication Protein A Antibody, clone RPA34-20 (mouse monoclonal antibody) validated in WB, ICC, IHC to detect Replication Protein A also known as Replication protein A 32 kDa subunit, RP-A p32.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Replication Protein A in 10 µg of HeLa cell lysate.

Target description

~30 kDa observed. Uniprot describes three isoforms at ~29 kDa, ~30 kDa, and ~39 kDa

Linkage

Replaces: NA19L

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate.B10

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Victor Chun-Lam Wong et al.
Cell cycle (Georgetown, Tex.), 11(13), 2526-2537 (2012-06-23)
We examined genotoxic signaling and cell fate decisions in response to a potent DNA-protein crosslinker formaldehyde (FA). DNA-protein crosslinks (DPC) are poorly understood lesions produced by bifunctional carcinogens and several cancer drugs. FA-treated human cells showed a rapid activation of
Yana P Blokhina et al.
Journal of visualized experiments : JoVE, (157) (2020-03-24)
Meiosis is the key cellular process required to create haploid gametes for sexual reproduction. Model organisms have been instrumental in understanding the chromosome events that take place during meiotic prophase, including the pairing, synapsis, and recombination events that ensure proper
Joëlle A Desmarais et al.
Stem cells (Dayton, Ohio), 30(7), 1385-1393 (2012-05-04)
Pluripotent cells of the early embryo, to which embryonic stem cells (ESCs) correspond, give rise to all the somatic cells of the developing fetus. Any defects that occur in their genome or epigenome would have devastating consequences. Genetic and epigenetic
Fiifi Neizer-Ashun et al.
Nucleic acids research, 50(19), 11028-11039 (2022-10-17)
The lysine-rich coiled-coil 1 (KRCC1) protein is overexpressed in multiple malignancies, including ovarian cancer, and overexpression correlates with poor overall survival. Despite a potential role in cancer progression, the biology of KRCC1 remains elusive. Here, we characterize the biology of
Akiko Tomita et al.
Nature communications, 14(1), 5607-5607 (2023-09-16)
CRISPR/Cas9-mediated gene editing has great potential utility for treating genetic diseases. However, its therapeutic applications are limited by unintended genomic alterations arising from DNA double-strand breaks and random integration of exogenous DNA. In this study, we propose NICER, a method

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