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MABF2791

Sigma-Aldrich

Anti-CCR9 Antibody, clone 7E7

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Synonym(s):
C-C chemokine receptor type 9;C-C CKR-9;CC-CKR-9;CCR-9;Chemokine C-C receptor 10;CDw199

biological source

rat

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

7E7, monoclonal

mol wt

calculated mol wt 41.91 kDa

purified by

using protein G

species reactivity

mouse, human

packaging

antibody small pack of 100 μg

technique(s)

flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

isotype

IgG2b

epitope sequence

N-terminal

Protein ID accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... TACSTD2(4070)

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This Item
MABF2814SAB4500329PRS1112
vibrant-m

MABF2791

Anti-CCR9 Antibody, clone 7E7

vibrant-m

MABF2814

Anti-CCR9 Antibody, clone 9B-1

biological source

rat

biological source

rat

biological source

rabbit

biological source

rabbit

clone

7E7, monoclonal

clone

9B-1, monoclonal

clone

polyclonal

clone

polyclonal

species reactivity

mouse, human

species reactivity

mouse

species reactivity

human

species reactivity

human

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

conjugate

unconjugated

isotype

IgG2b

isotype

IgG2aκ

isotype

-

isotype

-

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General description

C-C chemokine receptor type 9 (UniProt: Q9WUT7; also known as C-C CKR-9, CC-CKR-9, CCR-9, Chemokine C-C receptor 10, CDw199) is encoded by the Crr9 (also known as Cmkbr10) gene (Gene ID: 12769) in murine species. CCR9 is a multi-pass membrane protein of the G-protein coupled receptor 1 family. It has four extracellular domains, seven transmembrane domains, and four cytoplasmic domains and serves as a receptor for chemokine CCL25/TECK. It is highly expressed in the thymus tissue. It is also expressed on virtually all small intestinal T cells and IgA-producing plasma cells (PCs) from the spleen. It is reported to be crucial for the positioning of PCs to the small intestine. Higher expression of CCR9 is observed in newly generated PCs and it is down-regulated once these cells reach their final destination in the intestine. In wild-type mice, a majority of the PCs are shown to locate to the lower half of the villus where CCL25 is also expressed at high levels. CCR9 deficient mice exhibit reduced number of PCs in the intestine and are unable to mount a T cell dependent antigen specific IgA response. CCR9-/- hematopoietic stem cells are deficient in their ability to generate all major thymocyte subsets. (Ref.: Svensson, M., et al. (2008). J. Leukoc. Biol. 83(1); 156-164; Pabst, O., et al. (2004). J. Exp. Med. 199(3); 411-416).

Specificity

Clone 7E7 is a rat monoclonal antibody that detects C-C chemokine receptor type 9 (CCR9). It targets an epitope within 20 amino acids from the fist extracellular domain in the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 20 amino acids from the first extracellular domain in the N-terminal region of mouse C-C-chemokine receptor type 9 (CCR9).

Application

Quality Control Testing

Evaluated by Flow Cytometry in BALB/c mouse thymocytes.

Flow Cytometry Analysis: 1 μg of this antibody detected CCR9 in one million BALB/c mouse thymocytes.

Tested Applications

Immunohistochemistry Applications: A representative lot detected CCR9 in Immunohistochemistry applications (Guo, Z., et al. (2008). Int Immunol. 20(3):307-15; Pabst, O., et al. (2004). J Exp Med. 199(3):411-6; McGuire, H.M., et al. (2011). Immunity. 34(4):602-15).

Flow Cytometry Analysis: A representative lot detected CCR9 in Flow Cytometry applications (Guo, Z., et al. (2008). Int Immunol. 20(3):307-15; Pabst, O., et al. (2004). J Exp Med. 199(3):411-6; McGuire, H.M., et al. (2011). Immunity. 34(4):602-15; Svensson, M., et al. (2008). J Leukoc Biol. 83(1):156-64; Chennupati, V., et al. (2010). J Immunol. 185(9):5160-8).

Immunofluorescence Analysis: A representative lot detected CCR9 in Immunofluorescence applications (Guo, Z., et al. (2008). Int Immunol. 20(3):307-15; Pabst, O., et al. (2004). J Exp Med. 199(3):411-6; McGuire, H.M., et al. (2011). Immunity. 34(4):602-15).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-CCR9, clone 7E7, Cat. No. MABF2791, is a rat monoclonal antibody that detects CCR9 and is tested for use in Flow Cytometry, Immunofluorescence, and Immunohistochemistry.

Physical form

Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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