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SAB4501661

Sigma-Aldrich

Anti-RHOA antibody produced in rabbit

affinity isolated antibody

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Synonym(s):
ARH12, ARHA, ARHA2, H12, RHO12
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 21 kDa

species reactivity

rat, mouse, human

concentration

~1 mg/mL

technique(s)

ELISA: 1:1000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RHOA(387)

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This Item
SAB5701197HPA013440SAB1401016
antibody form

affinity isolated antibody

antibody form

-

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

conjugate

unconjugated

clone

polyclonal

clone

monoclonal

clone

polyclonal

clone

polyclonal

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

dry ice

form

buffered aqueous solution

form

liquid

form

buffered aqueous glycerol solution

form

buffered aqueous solution

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General description

Anti-RHOA Antibody detects endogenous levels of total RHOA protein.
RhoA (ras homolog family member A) belongs to the Rho GTPase family of proteins. It is located on human chromosome 3p21.31.

Immunogen

The antiserum was produced against synthesized peptide derived from human RhoA.

Immunogen Range: 144-193

Biochem/physiol Actions

In multiciliated epithelial cells, RhoA (ras homolog family member A) controls the actin network dynamics. It also modulates membrane protrusion and contractility in the cell body. RhoA is one of the essential factor for Ras transformation. It is associated with cancer metastasis.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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GPX1 (Glutathione Peroxidase 1)
Kulak MV and Weigel RJ
Atlas of Genetics and Cytogenetics in Oncology and Haematology (2014)
Activation of Rac1, RhoA, and mitogen-activated protein kinases is required for Ras transformation
Khosravi-Far R, et al.
Molecular and Cellular Biology, 15(11), 6443-6453 (1995)
Chia-Hsin Chan et al.
Nature cell biology, 12(5), 457-467 (2010-04-13)
The RhoA GTPase is crucial in numerous biological functions and is linked to cancer metastasis. However, the understanding of the molecular mechanism responsible for RhoA transcription is still very limited. Here we show that RhoA transcription is orchestrated by the
Variegated RHOA mutations in human cancers
Kataoka K and Ogawa S
Experimental Hematology, 44(12), 1123-1129 (2016)
Spatiotemporal dynamics of RhoA activity in migrating cells
Pertz O, et al.
Nature, 440(7087), 1069-1072 (2006)

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