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MABF473

Sigma-Aldrich

Anti-Ly-6G (Gr-1) Antibody (mouse), PE-Cy7, clone RB6-8C5

clone RB6-8C5, 0.2 mg/mL, from rat

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Synonym(s):
Lymphocyte antigen 6G, Ly-6G, Ly-6G.1
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

conjugate

PE-Cy7

antibody form

purified antibody

antibody product type

primary antibodies

clone

RB6-8C5, monoclonal

species reactivity

mouse

concentration

0.2 mg/mL

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG2bκ

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Ly6G(546644)

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This Item
MABF472MABF474MABF147E
species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

conjugate

PE-Cy7

conjugate

PE

conjugate

-

conjugate

-

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

Gene Information

mouse ... Ly6G(546644)

Gene Information

mouse ... Ly6G(546644)

Gene Information

mouse ... Ly6G(546644)

Gene Information

mouse ... Ly6G(546644)

clone

RB6-8C5, monoclonal

clone

RB6-8C5, monoclonal

clone

RB6-8C5, monoclonal

clone

RB6-8C5, monoclonal

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General description

The RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports sµggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S. and Schlueter AJ. 2003. Cytometry A, 58(2): 195-200.
This rat monoclonal antibody Anti-Ly-6G (Gr-1) (mouse), PE-Cy7, clone RB6-8C5 validated for use in IHC, Flow Cytometry & IP for the detection of Ly-6G (Gr-1).

Quality

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.25 µg of this antibody detected Ly-6G (Gr-1) in one million C57Bl/6 bone marrow cells.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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