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MABF80A

Sigma-Aldrich

Anti-CD11b, (human/mouse), FITC, clone M1/70 Antibody

clone M1/70, from rat, FITC conjugate

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Synonym(s):
Integrin alpha-M, CD11 antigen-like family member B, CR-3 alpha chain, Cell surface glycoprotein MAC-1 subunit alpha, Leukocyte adhesion receptor MO1, CD11b, CD11b,
eCl@ss:
32160702
NACRES:
NA.44

biological source

rat

Quality Level

conjugate

FITC conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

M1/70, monoclonal

species reactivity

human, mouse

packaging

antibody small pack of 25 μg

technique(s)

flow cytometry: suitable

isotype

IgG2bκ

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... ITGAM(3684)

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MABF515MABF365MABF514
conjugate

FITC conjugate

conjugate

PE-Cy5

conjugate

PE-Cy7

conjugate

biotin conjugate

biological source

rat

biological source

rat

biological source

rat

biological source

rat

clone

M1/70, monoclonal

clone

M1/70, monoclonal

clone

M1/70, monoclonal

clone

M1/70, monoclonal

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

Gene Information

human ... ITGAM(3684)

Gene Information

human ... ITGAM(3684)

Gene Information

human ... ITGAM(3684)

Gene Information

human ... ITGAM(3684)

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General description

The M1/70 antibody reacts with human and mouse CD11b, also known as integrin alpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin receptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrins are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin receptors consist of distinct combinations of integrin chains which are differentially expressed. Integrin alpha M (CD11b) assembles with Integrin beta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling through ICAM-1 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.The M1/70 antibody is widely used as a marker for CD11b expression on mouse macrophages, granulocytes, neutrophils, and NK cells. The antibody is also reported to be cross-reactive for Rhesus macaque CD11b.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This rat monoclonal Anti-CD11b, Antibody (human/mouse), FITC, clone M1/70, Cat. No. MABF801 is validated for use in Flow Cytometry, for the detection of CD11b.

Quality

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 μg from a representative lot detected CD11b, in one million C57Bl/6 bone marrow cells.

Physical form

Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Purified by size-exclusion chromatography

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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