MilliporeSigma
All Photos(2)

Documents

MABN1194

Sigma-Aldrich

Anti-PSD95 Antibody, clone 3H4.3

clone 3H4.3, from mouse

Sign Into View Organizational & Contract Pricing

Synonym(s):
Disks large homolog 4, PSD95, Postsynaptic density protein 95, SAP90
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3H4.3, monoclonal

species reactivity

mouse, rat, human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... DLG4(1742)
rat ... Dlg4(29495)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
P246ABN334MABN1190
Anti-PSD95 Antibody, clone 3H4.3 clone 3H4.3, from mouse

MABN1194

Anti-PSD95 Antibody, clone 3H4.3

Anti-SAPAP4 Antibody from rabbit, purified by affinity chromatography

ABN334

Anti-SAPAP4 Antibody

Anti-PSD95 Antibody, clone 1A8.1 clone 1A8.1, from mouse

MABN1190

Anti-PSD95 Antibody, clone 1A8.1

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

mouse

clone

3H4.3, monoclonal

clone

7E3-1B8, monoclonal

clone

polyclonal

clone

1A8.1, monoclonal

species reactivity

mouse, rat, human

species reactivity

rat

species reactivity

rat, human

species reactivity

rat, human

Gene Information

human ... DLG4(1742)
rat ... Dlg4(29495)

Gene Information

rat ... Dlg4(29495)

Gene Information

human ... DLGAP4(22839)

Gene Information

human ... DLG4(1742)

UniProt accession no.

P31016

UniProt accession no.

P31016

UniProt accession no.

Q9Y2H0

UniProt accession no.

P78352

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Disks large homolog 4 (UniProt: P31016; also known as Postsynaptic density protein 95, PSD-95, Synapse-associated protein 90, SAP-90, SAP90) is encoded by the Dlg4 (also known as Dlgh4, Psd95) gene (Gene ID: 29495) in rat species. PSD95 belong to the family of membrane-associated guanylate kinases (MAGUKs), which also include PSD-93, SAP97 and SAP102. PSD95 is predominantly expressed in the hippocampus CA1 region and prefrontal cortex of the brain, where it localizes in a somatodendritic pattern in the post-synaptic membrane and in presynaptic axon terminals of inhibitory neurons. PDS95 is the major scaffolding protein in the excitatory postsynaptic density (PSD) and a potent regulator of synaptic strength via interactions with a diverse PSD proteins, including NMDA receptors (NMDARs), AMPA receptor (AMPAR) complexes via Stargazin/TARP, adhesion molecules, as well as other scaffolding proteins, such as GKAP and Shank. PSD95 and neuronal nitric-oxide synthase (nNOS) interaction plays a role in maintaining hypersensitivity in acute and chronic pain. The assembly of the GluR6-PSD95-CaMKII complex is reported to mediate injury induced by brain ischemia by facilitating GluR6 serine phosphorylation by CaMKII and the activation of JNK. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons.

Specificity

Clone 3H4.3 detects PSD95 in human and mouse brain.

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of rat PSD95 protein.

Application

Anti-PSD95, clone 3H4.3, Cat. No. MABN1194, is a highly specific mouse monoclonal antibody that targets PSD95 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PSD95 in human cerebellum, rat cerebellum, and rat brain stem tissues.
Research Category
Neuroscience

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PSD-95 in 10 µg of mouse brain tissue lysate.

Target description

~95 kDa observed; 80.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Peng Chen et al.
Cell death & disease, 12(4), 403-403 (2021-04-16)
The genes encoding for neuregulin1 (NRG1), a growth factor, and its receptor ErbB4 are both risk factors of major depression disorder and schizophrenia (SZ). They have been implicated in neural development and synaptic plasticity. However, exactly how NRG1 variations lead
Shatrunjai Giri et al.
Journal of neuroscience research, 99(7), 1815-1834 (2021-04-06)
Rapid eye movement sleep (REMS) favors brain development and memory, while it is decreased in neurodegenerative diseases. REMS deprivation (REMSD) affects several physiological processes including memory consolidation; however, its detailed mechanism(s) of action was unknown. REMS reduces, while REMSD elevates

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service