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  • Rapid eye movement sleep deprivation impairs neuronal plasticity and reduces hippocampal neuronal arborization in male albino rats: Noradrenaline is involved in the process.

Rapid eye movement sleep deprivation impairs neuronal plasticity and reduces hippocampal neuronal arborization in male albino rats: Noradrenaline is involved in the process.

Journal of neuroscience research (2021-04-06)
Shatrunjai Giri, Amit Ranjan, Awanish Kumar, Megha Amar, Birendra Nath Mallick
ABSTRACT

Rapid eye movement sleep (REMS) favors brain development and memory, while it is decreased in neurodegenerative diseases. REMS deprivation (REMSD) affects several physiological processes including memory consolidation; however, its detailed mechanism(s) of action was unknown. REMS reduces, while REMSD elevates noradrenaline (NA) level in the brain; the latter induces several deficiencies and disorders, including changes in neuronal cytomorphology and apoptosis. Therefore, we proposed that REMS- and REMSD-associated modulation of NA level might affect neuronal plasticity and affect brain functions. Male albino rats were REMS deprived by flower-pot method for 6 days, and its effects were compared with home cage and large platform controls as well as post-REMSD recovered and REMS-deprived prazosin (α1-adrenoceptor antagonist)-treated rats. We observed that REMSD reduced CA1 and CA3 neuronal dendritic length, branching, arborization, and spine density, while length of active zone and expressions of pre- as well as post-synaptic proteins were increased as compared to controls; interestingly, prazosin prevented most of the effects in vivo. Studies on primary culture of neurons from chick embryo brain confirmed that NA at lower concentration(s) induced neuronal branching and arborization, while higher doses were destructive. The findings support our contention that REMSD adversely affects neuronal plasticity, branching, and synaptic scaffold, which explain the underlying cytoarchitectural basis of REMSD-associated patho-physio-behavioral changes. Consolidation of findings of this study along with that of our previous reports suggest that the neuronal disintegration could be due to either withdrawal of direct protective and proliferative role of low dose of NA or indirect effect of high dose of NA or both.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Rabbit Anti-Chicken IgG Antibody, HRP conjugate, 0.8 mg/mL, Chemicon®
Sigma-Aldrich
Anti-PSD95 Antibody, clone 3H4.3, clone 3H4.3, from mouse
Sigma-Aldrich
Anti-MAP2 Antibody, Alexa Fluor 488 conjugated, clone AP20, Chemicon®, from mouse
Sigma-Aldrich
Anti-phospho-Munc-18 (Ser515) Antibody, Chemicon®, from rabbit
Sigma-Aldrich
Anti-GAPDH Antibody, from chicken, purified by affinity chromatography
Sigma-Aldrich
DAPI, for nucleic acid staining
Sigma-Aldrich
Prazosin hydrochloride, ≥99.0% (HPLC)