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MABN1846

Sigma-Aldrich

Anti-Apelin Receptor/APJ, clone 3C3-7 Antibody

clone 3C3-7, from mouse

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Synonym(s):
Apelin receptor, Angiotensin receptor-like 1, G-protein coupled receptor APJ, G-protein coupled receptor HG11
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3C3-7, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... APLNR(187)

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Anti-GPR Antibody3 from rabbit, purified by affinity chromatography

Sigma-Aldrich

ABN473

Anti-GPR Antibody3

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

clone

3C3-7, monoclonal

clone

polyclonal

clone

8B7.1, monoclonal

clone

polyclonal

species reactivity

human

species reactivity

human

species reactivity

mouse, human, rat

species reactivity

mouse, human

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

ELISA: 1:10000, immunofluorescence: 1:100-1:500, western blot: 1:500-1:1000

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunofluorescence: suitable, immunohistochemistry: suitable, western blot: suitable

isotype

IgG1κ

isotype

-

isotype

IgG2a

isotype

-

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General description

Apelin receptor (UniProt P35414; also known as Angiotensin receptor-like 1, G-protein coupled receptor APJ, G-protein coupled receptor HG11) is encoded by the APLNR (also known as AGTRL1, APJ) gene (Gene ID 187) in human. Apelin is an angiotensin converting enzyme 2 (ACE2) substrate that functions as an inotropic and cardiovascular protective peptide. Apelin exerts its physiological effects via its receptor APJ, a seven-transmembrane G protein-coupled receptor that shares significant homology with the Ang II type 1 receptor (AT1R). Apelin/APJ signaling plays an important role in a wide range of pathophysiological effects in the cardiovascular system. Apelin levels are reduced in patients with essential hypertension independent of left ventricular systolic and diastolic dysfunction. Treatment with Apelin has been shown to lower systolic blood pressure levels in hypertensive rats via an NO-dependent mechanism. APJ is a 7-transmembrane (a.a. 27-51, 67-91, 101-125, 145-166, 201-221, 245-271, 285-308) GPCR, having a short N-terminal extracellular portion (a.a. 1-26) and a long C-terminal cytomplasmic tail (a.a. 309-380).

Specificity

Clone 3C3-7 detects human Apelin receptor/APJ.

Immunogen

Recombinant full-length human Apelin receptor/APJ.

Application

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Apelin receptor/APJ in human prostate cancer tissue, as well as normal cerebral cortex and pancreas tissue sections.
Research Category
Neuroscience
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
This Anti-Apelin Receptor, clone 3C3-7 Antibody is validated for use in Immunohistochemistry (Paraffin), Western Blotting for the detection of APJ.

Quality

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Apelin receptor/APJ in 10 µg of MCF7 cell lysate.

Target description

~42 kDa 42.66 kDa calculated. Uncharacterized band(s) may appear in some lysates.

Physical form

Format: Purified
Protein G purified.
Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Sahil Gulati et al.
Nature communications, 9(1), 1996-1996 (2018-05-20)
G protein-coupled receptors (GPCRs) activate heterotrimeric G proteins by mediating a GDP to GTP exchange in the Gα subunit. This leads to dissociation of the heterotrimer into Gα-GTP and Gβγ dimer. The Gα-GTP and Gβγ dimer each regulate a variety

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