MABN844
Anti-Neurophysin 1 Antibody, clone PS 38
clone PS 38, from mouse
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Synonym(s):
Oxytocin-neurophysin 1, OT-NPI, Oxytocin, Ocytocin, Neurophysin 1
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
PS 38, monoclonal
species reactivity
rat
technique(s)
immunohistochemistry: suitable
radioimmunoassay: suitable
western blot: suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
rat ... Oxt(25504)
General description
Neurophysins are linear cycteine-rich proteins containing 93-95 amino acids. Two types of neurophysins, NPI and NPII are found in most mammalian species and they serve as carriers of oxytocin (OT) and vasopressin (AVP), respectively. Each NP and its associated hormone is synthesized as one precursor protein, OT-NPI (UniProt P01179) encoded by the Oxt (or Ot) gene or VAP-NPII (UniProt P01186) encoded by the Avp gene in rat species. Post-translational cleavages remove the signal peptide and process the precursor into mature OT and NPI (aa 20-28 and aa 32-135, respectively, of OT-NPI), or AVP and NPII (aa 24-32 and aa 36-128, respectively, of AVP-NPII). OT is a potent hormone and neurotransmitter secreted within the pituitary gland. OT exerts its biological activity through its receptor OXTR (also known as OTR). OT is used clinically for labor induction and may exhibit therapeutic potential for treating social anxiety disorders. VAP functions primarily as an anti-diuretic and regulates the concentration of water, glucose, and salts in blood. VAP is secreted from the posterior pituitary, but exerts its biological activity in the kidney via the G protein-coupled receptor AVPR2 (V2R).
Specificity
Clone PS 38 detects neurophysin 1 (oxytocin-associated NP), but not ocytocin/oxytocin or neurophysin 2 (vasopressin-associated NP)
Immunogen
Epitope: Neurophysin 1
KLH-conjugated acid soluble extract of rat (Sprague-Dawley) intermediate lobes corresponding to rat Neurophysin 1.
Application
Immunohistochemistry: A representative lot detected oxytocin (OT)-synthesizing neurons in the hypothalamic supraoptic (SON) and neural lobe (NL) regions of paraffin-embedded rat hypothalamus and pituitary tissue sections (Ben-Barak, Y., et al. (1985). J. Neurosci. 5(1):81-97).
Radioimmunoassay (RIA): A representative lot was assessed for binding affinity toward purified rat neurophysinn 1 by RIA (Ben-Barak, Y., et al. (1985). J. Neurosci. 5(1):81-97).
Western Blotting Analysis: A representative lot detected neurophysinn 1 in rat posterior pituitary extracts (Ben-Barak, Y., et al. (1985). J. Neurosci. 5(1):81-97).
Radioimmunoassay (RIA): A representative lot was assessed for binding affinity toward purified rat neurophysinn 1 by RIA (Ben-Barak, Y., et al. (1985). J. Neurosci. 5(1):81-97).
Western Blotting Analysis: A representative lot detected neurophysinn 1 in rat posterior pituitary extracts (Ben-Barak, Y., et al. (1985). J. Neurosci. 5(1):81-97).
Research Category
Neuroscience
Neuroscience
Research Sub Category
Developmental Signaling
Developmental Signaling
This Anti-Neurophysin 1 Antibody, clone PS 38 is validated for use in Western Blotting, Immunohistochemistry, Radioimmunoassay for the detection of Neurophysin 1.
Quality
Evaluated by Western Blotting in rat pituitary tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Neurophysin 1 in 10 µg of rat pituitary tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Neurophysin 1 in 10 µg of rat pituitary tissue lysate.
Target description
~12 kDa observed
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Dipanwita Pati et al.
Journal of neuroendocrinology, 32(3), e12839-e12839 (2020-03-07)
Significant prior evidence indicates that centrally acting oxytocin robustly modulates stress responsiveness and anxiety-like behaviour, although the neural mechanisms behind these effects are not entirely understood. A plausible neural basis for oxytocin-mediated stress reduction is via inhibition of corticotrophin-releasing hormone
Marie Habart et al.
STAR protocols, 4(1), 101968-101968 (2023-01-05)
Here, we present an optimized iDISCO+ protocol combining tissue clearing and light sheet microscopy to map the postnatal development of oxytocin and vasopressin neurons in mouse hypothalamus. We describe tissue preparation, immunostaining, clearing, and imaging. We then detail how to
Hongyang Wang et al.
Neuroendocrinology, 113(3), 343-360 (2022-09-01)
In the regulation of oxytocin (OT) neuronal activity, hydrogen sulfide (H2S), a gaseous neurotransmitter, likely exerts an excitatory role. This role is associated with increased expression of astrocytic cystathionine-β-synthase (CBS), the key enzyme for H2S synthesis. However, it remains unclear
Hanna Hörnberg et al.
Nature, 584(7820), 252-256 (2020-08-08)
A fundamental challenge in developing treatments for autism spectrum disorders is the heterogeneity of the condition. More than one hundred genetic mutations confer high risk for autism, with each individual mutation accounting for only a small fraction of cases1-3. Subsets
Fei Zeng et al.
ASN neuro, 13, 17590914211055064-17590914211055064 (2021-11-24)
This study investigated the effects of the pharmacological manipulation of noradrenergic activities on dopaminergic phenotypes in aged rats. Results showed that the administration of L-threo-3,4-dihydroxyphenylserine (L-DOPS) for 21 days significantly increased the expression of tyrosine hydroxylase (TH) and dopamine transporter
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