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MABS1915M

Sigma-Aldrich

Anti-EGFRvIII Antibody, clone DH8.3

clone DH8.3, from mouse

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Synonym(s):
Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, Epidermal growth facttor receptor vIII
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

DH8.3, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μg

technique(s)

ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... EGFR(1956)

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This Item
MABS835MABS829SAB4500711
Anti-EGFR Antibody, clone 7F12.1 clone 7F12.1, from mouse

MABS835

Anti-EGFR Antibody, clone 7F12.1

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

rat, human, mouse

Gene Information

human ... EGFR(1956)

Gene Information

human ... EGFR(1956)

Gene Information

human ... EGFR(1956)

Gene Information

human ... EGFR(1956)

clone

DH8.3, monoclonal

clone

7F12.1, monoclonal

clone

3E7.2, monoclonal

clone

polyclonal

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable, flow cytometry: suitable, immunoprecipitation (IP): suitable

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

ELISA: 1:40000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

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General description

Epidermal growth factor receptor (UniProt: P00533; also known as EC:2.7.10.1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1) is encoded by the EGFR (also known as ERBB, ERBB1, HER1) gene (Gene ID: 1956) in human. EGFR is a single-pass type 1 membrane protein that is ubiquitously expressed. It is synthesized with a 24 amino acids signal peptide that is subsequently cleaved. It contains an extracellular domain (aa 25-645) a transmembrane domain (aa 646-668), and a cytoplasmic domain (aa 669-1210). Upon binding of EGF, the receptor is phosphorylated and recruits adapter proteins to turn on downstream signaling cascades. EGFR is commonly overexpressed and is mutated in many human malignancies and is often associated with aggressive phenotypes. The most common truncated EGFR is the variant III EGFR deletion mutant (EGFRvIII), containing an inframe deletion of exons 2 7 (801 bp) from the extracellular domain. EGFRvIII mutation is reported in about 5% of human lung squamous cell carcinoma (SCC). It has also been suggested that EGFRvIII potentially plays an essential role in the human NSCLC tumor initiation and maintenance. Drugs such as Gefitinib, erlotnib, and HKI-272 are shown to inhibit the growth of EGFRvIII transformed cells and tumor growth. (Ref.: Ji, H., et al. (2006). Proc. Natl. Acad. Sci. USA 103(20); 7817-7822).

Specificity

Clone DH8.3 detects EGFR variant vIII a truncated form of EGFR, present in human cancer cells.

Immunogen

KLH-conjugated linear peptide corresponding to EGFRvIII (EGFR variant III) (LEEKKGNYVVTDHC) sequence.

Application

Anti-EGFRvIII, clone DH8.3, Cat. No. MABS1915, is a mouse monoclonal antibody that specifically detects epidermal growth factor receptor vIIIa and has been tested for use in ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
Research Category
Signaling
Western Blotting Analysis: A representative lot detected EGFRvIII in Western Blotting applications (Lammering, G., et. al. (2004). Clin Cancer Res. 10(19):6732-43; Nishikawa, R., et. al. (2004). Brain Tumor Pathol. 21(2):53-6; Hills, D., et. al. (1995). Int J Cancer. 63(4):537-43).

Flow Cytometry Analysis: A representative lot detected EGFRvIII in Flow Cytometry applications (Johns, T.G., et. al. (2002). Int J Cancer. 98(3):398-408; Hills, D., et. al. (1995). Int J Cancer. 63(4):537-43).

Immunohistochemistry Analysis: A representative lot detected EGFRvIII in Immunohistochemistry applications (Jungbluth, A.A., et. al. (2003). Proc Natl Acad Sci USA. 100(2):639-44; Nishikawa, R., et. al. (2004). Brain Tumor Pathol. 21(2):53-6; Feng, H., et. al. (2014). J Clin Invest. 124(9):3741-56; Feng, H., et. al. (2012). Proc Natl Acad Sci USA. 109(8):3018-23.

Immunoprecipitation Analysis: A representative lot detected EGFRvIII in Immunoprecipitation applications (Lammering, G., et. al. (2004). Clin Cancer Res. 10(19):6732-43; Hills, D., et. al. (1995). Int J Cancer. 63(4):537-43).

ELISA Analysis: A representative lot detected EGFRvIII in ELISA applications (Johns, T.G., et. al. (2002). Int J Cancer. 98(3):398-408; Hills, D., et. al. (1995). Int J Cancer. 63(4):537-43).

Quality

Evaluated by Western Blotting in U87MG.∆EGFR cell lysates.

Western Blotting Analysis: 4 ug/mL of this antibody detected EGFRvIII in 10 µg of U87MG.∆EGFR cell lysates.

Target description

~140 kDa observed; 134.28 kDa calculalted. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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