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MABS445

Sigma-Aldrich

Anti-Sclerostin Antibody, clone 7B6.1 | MABS445

culture supernatant, clone 7B6.1, from mouse

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Synonym(s):
Sclerostin
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

culture supernatant

antibody product type

primary antibodies

clone

7B6.1, monoclonal

species reactivity

rat, human

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... SOST(50964)

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SAB1300753SAB1307103SAB1306836
antibody form

culture supernatant

antibody form

Ig fraction of antiserum

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

clone

7B6.1, monoclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

rat, human

species reactivity

human

species reactivity

mouse, human

species reactivity

human, mouse

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: 1:50-1:100, indirect ELISA: 1:1000

technique(s)

immunohistochemistry: 1:10-1:50, western blot: 1:1000

technique(s)

western blot: 1:1000

isotype

IgG2bκ

isotype

-

isotype

-

isotype

-

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General description

Sclerostin (UniProt Q9BQB4) is encoded by the SOST (also known as CDD, SOST1, VBCH) gene (ORP UNQ2976/PRO7455/PRO7476; Gene ID 50964) in human. Sclerostin is a glycoprotein expressed in osteocytes and functions as a negatively regulator against bone mass by antagonizing low density lipoprotein receptor-related proteins- (LRP5-/LRP6-) mediated WNT/beta-catenin pathway signaling. Sclerostin levels are increased in the post-menopausal women and elderly and in long term immobilized patients. Sclerostin neutralizing antibody is reported to enhance bone formation, mass, and strength in aged male rats. Sclerostin and its inhibitors have potential in prosthetic, regenerative and preventive therapy in dentistry.

Specificity

Detects Sclerostin isoform 1 (UniProt Q9BQB4-1). Reactivity toward the spliced isoform 2 (UniProt Q9BQB4-2) is likely, but has not been determined.

Immunogen

Epitope: Internal
GST-tagged recombinant protein corresponding to the internal fragment of human Sclerostin.

Application

Anti-Sclerostin Antibody, clone 7B6.1 | MABS445 is an antibody against Sclerostin Antibody for use in Western Blotting and Immunohistochemistry.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Sclerostin in human kidney, rat liver and human liver tissue.
Research Category
Signaling
Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blotting in GST-tagged Sclerostin recombinant protein.

Western Blotting Analysis: A 1:500 dilution of this antibody detected 0.5 µg of GST-tagged Sclerostin recombinant protein.

Target description

24 kDa calculated

Physical form

Mouse monoclonal IgG2bκ antibody tissue culture supernatant without preservatives.
Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Richard J Visconti et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 36(10), 1914-1930 (2021-06-27)
Human myeloma bone disease (MBD) occurs when malignant plasma cells migrate to the bone marrow and commence inimical interactions with stromal cells, disrupting the skeletal remodeling process. The myeloma cells simultaneously suppress osteoblastic bone formation while promoting excessive osteoclastic resorption.

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